摘要
研究幽门螺杆菌细胞毒素相关基因A(HpcagA)在胃粘膜恶变过程中的作用 ,以探讨在胃癌发生过程中的分子学机制。方法 :16 3例Hp阳性患者 (慢性浅表性胃炎和萎缩性胃炎各 30例 ,肠上皮化生 33例 ,不典型增生 31例、胃癌 39例 ) ,用原位聚合酶链反应 (PCR)、常规PCR和原位杂交等方法检测胃粘膜HpcagA。结果 :16 3例标本中 ,原位PCRHpcagA阳性率为 5 6 .4% ,显著高于原位杂交 (44 .8% ) ,但与常规PCR 5 2 .1%的阳性率相比二者无明显差异。原位PCR检出HpcagA阳性率从浅表性胃炎、癌前病变到胃癌逐渐升高。Hpca gA阳性信号可出现在胃癌及癌前病变组织和胃粘膜上皮与腺上皮中 ,部分病例阳性信号甚至出现于上皮细胞核内。结论 :HpcagA可能有助于胃粘膜组织的恶性转化 ,并可通过刺激胃粘膜细胞增殖、使癌基因激活和抑癌基因失活而促进胃癌的发生。
Objective: To study the effects of Helicobacter pylori (Hp) cytotoxin associated gene A (cagA) on the canceration of gastric mucosa, and the possible role of HpcagA infection in the carcinogenesis of gastric cancer (GC). Method: A total of 163 patients were inspected by a non-radioactive in situ PCR method for the detection of HpcagA and it had been compared with conventional methods for diagnosis of the infection. Results: compared with in situ hybridization (44.8%) and common PCR (52.1%), in situ PCR has the highest HpcagA positive rate (56.4%). Higher occurrence of HpcagA infection was found in patients with GC and precancerous lesion than that in patients with CSG (P<0.05). HpcagA was located not only in surface mucous gellayer, but also within gastric epithelial cell nuclears in patient with GC and premalignant lesions. Conclusions: The infection of HpcagA may promote gastric mucosa to become cancerous and these processes may have much to do with the HpcagA located within epithelial cell nuclears.theinfection .Results :c
出处
《中国内镜杂志》
CSCD
2001年第4期43-44,46,共3页
China Journal of Endoscopy
