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一个新的水稻GST类似蛋白基因XIG的克隆与分析 被引量：1

Cloning and Analysis of the Rice Gene Coding a New GST-like Protein
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摘要 根据编码一种玉米GST类似蛋白的mRNAIn2 1序列设计引物 ,以水稻cDNA文库为模板 ,PCR扩增得到一条 2 70bp的DNA片段 .以此片段为探针分别筛选水稻根cDNA文库及水稻基因文库 ,获得一条全长cDNA及其相应的全长基因 ,并通过测序得到了两者的全序列 .该基因编码的蛋白具有GST的典型特征 。 A pair of primers were designed according to the sequence of In 2 1 mRNA that was coding a GST like protein in Maize. A DNA fragment of 270 base pairs was amplified from a rice root cDNA library by the means of PCR. Using the PCR fragment as the probe for screening, a complete cDNA and its complete gene were obtained from that cDNA library and a rice gene library, respectively. Both of the complete cDNA and the complete gene were sequenced. The protein, coded by the newly reported rice gene, showed typical characteristics of GSTs.
作者 张翼飞 史晰 王喜萍 曹凯鸣
机构地区 复旦大学生命科学学院 上海 上海
出处 《复旦学报（自然科学版）》 CAS CSCD 北大核心 2002年第1期27-30,35,共5页 Journal of Fudan University：Natural Science
基金 上海市现代生物与新药产业发展基金 (994 3192 15 1) 国家转基因植物研究与产业化基金 (J99 A 0 4 4 )
关键词 水稻 谷胱甘肽-S-转移酶 蛋白质结构 rice GST protein structure
分类号 Q751 [生物学—分子生物学] S511 [农业科学—作物学]
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参考文献8

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同被引文献21

  • 1Dixon D P,Cole D J,Edwards R.Characterisation of a zeta class glutathione transferase from Arabidopsis thaliana with a putative role in tyrosine catabolism[J].Arch Biochem Biophys,2000,384(2):407-412.
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  • 3Loyall L,Uchida K,Braun S,et al.Frohnmeyer H.Glutathione and a UV light-induced glutathione S-transferase are involved in signaling to chalcone synthase in cell cultures[J].Plant Cell,2000,12(10):1939-1950.
  • 4Kampranis S C,Damianova R,Atallah M,et al.A novel plant glutathione S-transferase/peroxidase suppresses Bax lethality in yeast[J].J Biol Chem,2000,275(38):29207-29216.
  • 5Dixon D P,Davis B G,Edwards R.Functional divergence in the glutathione transferase superfamily in plants.Identification of two classes with putative functions in redox homeostasis in Arabidopsis thaliana[J].J Biol Chem,2002,277(34):30859-30869.
  • 6McGonigle B,Keeler S J,Lau S M,et al.A genomics approach to the comprehensive analysis of the glutathione S-transferase gene family in soybean and maize[J].Plant Physiol,2000,124(3):1105-1120.
  • 7Wagner U,Edwards R,Dixon D P,et al.Probing the diversity of the Arabidopsis glutathione S-transferase gene family[J].Plant Mol Biol,2002,49(5):515-532.
  • 8Moons A.Osgstu3 and osgstu4,encoding tau class glutathione S-transferases,are heavy metal-and hypoxic stress-induced and differentially salt stress-responsive in rice roots[J].FEBS Lett,2003,553(3):427-432.
  • 9Soranzo N,Sari Gorla M,Mizzi L,et al.Organisation and structural evolution of the rice glutathione S-transferase gene family[J].Mol Genet Gen,2004,271(5):511-512.
  • 10Murashige T,Skoog F.A revised medium for rapid growth and bioassays with tobacco tissue cultures[J].Physiol Plant,1962,15(3):493-497.

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复旦学报（自然科学版）
2002年 第1期

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