摘要
目的研究骨髓基质细胞（ＢＭＳＣ）的成骨及软骨分化功能。方法 采用细胞静置贴壁培养法，体外培养了兔和儿童的ＢＭＳＣ，用传至２－３代兔ＢＭＳＣ和Ⅱ型胶原联合治疗兔膝关节软骨缺损；采用成骨诱导培养法研究儿童ＢＭＳＣ的成骨效应。结果（１）Ⅱ型胶原和ｂＦＧＦ对兔ＢＭＳＣ的贴壁和生长是有效的。（２）用兔ＢＭＳＣ和Ⅱ型胶原治疗１２周后，能使兔膝关节软骨缺损获得恢复，其软骨细胞分化明显。（３）用成骨诱导培养法，可使儿童ＢＭＳＣ骨钙素分泌水平比常规对照组升高８～１０倍，并可见有典型的成骨化的黑色矿化细胞区。结论骨髓基质细胞具有成骨和软骨分化功能。
Aim To study osteogenic and cartilaginous differentiation of bone marrow stromal cells (BMSC). Methods (1) The BMSC of children and rabbits were cultured in vitro by satic adherent assay. (2) The rabbit BMSC at passages 2~3 added in collagen-Ⅱ gel were transplanted into articular cartilage defects of rabbit knees for treatment. (3) The osteogenic effect of children ‘sBMSC were re- searched by using osteogenic induce culture in vitro. Results (1) The collagen-Ⅱ gel and bFGF were efficient for adherence and growth of rabbit BMSC. (2) After treatment for 12 weeks with BM- SC and collagen-Ⅱ gel, the articular cartilaginous defects of rabbit knees were recovered by cartilaginous cell differentiation. of BMSC. (3) Using induce culture assay of osteogenic cells, the level of teocacin expressed by children BMSC of experiment groups were 8 ~10 folds higher than that of control groups Mineralization of osteo genie cells could be seen under microscopy and electron microsco- py. Conclusion The BMSC have osteogenic and cartilaginous dif- ferentiation potential.
出处
《细胞与分子免疫学杂志》
CAS
CSCD
北大核心
2002年第1期80-82,共3页
Chinese Journal of Cellular and Molecular Immunology
关键词
骨髓基质细胞
成骨分化
软骨缺损治疗
软骨分化
bone marrow stromal cell
osteogenic differentiation
treatment of cartilage defect
cartilaginous differentia- tion
