摘要
目的 :探讨胆红素和胆汁酸是否通过调节尿苷二磷酸葡萄糖醛酸基转移酶 (UGT)的表达来加速自身的代谢。方法 :应用原始鼠肝细胞培养和Northen斑迹杂交技术 ,观察胆红素和胆汁酸处理肝细胞前后UGT同工酶mRNA的变化。结果 :用胆红素处理肝细胞 2 4h ,UGT1A1和UGT1A5mRNA的表达显著增加 ,分别为对照组的 16 3%和 133%。当肝细胞暴露于胆酸、鹅脱氧胆酸、脱氧胆酸、猪脱氧胆酸和石胆酸时 ,UGT2B1mRNA的表达水平明显增高 ,分别为对照组的 15 4 %、16 0 %、138%、15 3%和 149%。猪脱氧胆酸亦增加UGT2B3mRNA的表达。结论 :胆红素和胆汁酸可调节UGT的表达 ,这种调节可能会在清除病理性增高的胆红素和胆汁酸中起代偿作用。
Objective: To investigate whether bilirubin and bile acids could modulate their own metabolism by regulating UDP Glucuronosyltransferase (UGT) levels in cultured rat hepatocytes. Methods:Primary rat hepatocyte cultures and northern blot analysis have been used to determine the mRNA levels of five UGT isoforms in cultured hepatocytes following treatment with bilirubin and bile acids. Results:Incubation of hepatocytes with bilirubin (48 μmol) for 24 hours significantly increased the mRNA expression of UGT1A1 and UGT1A5 by163% and 133% respectively. Treatment with chenodeoxycholic acid, cholic acid, deoxycholic acid, hyodeoxycholic acid and lithocholic acid at a concentration of 100 μmol for 48 hours significantly enhanced the mRNA expression of UGT2B1 by 154%?160%?138%?153% and 149% respectively. UGT2B3 mRNA level was also increased by hyodeoxycholic acid. Conclusion:Bilirubin and bile acids can regulate UGT expression and such regulation could play a compensatory role in the elimination of the pathologically increased concentrations of these compounds in some hepatobiliary diseases.
出处
《山东大学学报（医学版）》
CAS
2002年第2期138-140,共3页
Journal of Shandong University:Health Sciences
关键词
胆红素
胆汁酸
葡糖醛酸基转移酶
肝细胞培养
bilirubin
bile acids
UDP Glucuronosyltransferase
hepatocyte cultures
