摘要
目的提纯角膜内皮抗原(cornealendothelialantigen,CEN-Ag)及抗角膜内皮抗原抗体(anti-cornealendothelialantibody,aCEN-Ab)。方法用猪角膜内皮做抗原,上皮做对照,经离子交换层析及2B4.14.1单克隆抗体亲和层析制得纯化的CEN-Ag和角膜上皮抗原(cornealepithelialantigen,CEP-Ag),用提纯的CEN-Ag和CEP-Ag分别免疫家兔,取血清经亲和层析得到aCEN-Ab和抗角膜上皮抗原抗体(anti-cornealepithelialantibody,aCEP-Ab)。结果经聚丙烯酰胺凝胶电泳(SDS-PAGE)分析,抗原CEN-Ag和CEP-Ag的Mr分别为43000和53000,相应的免疫球蛋白是IgG2和IgG3。
Objective To explorethemethodsforpurifyingcornealendothelialantigen(CEN-Ag)andpreparinganti-corneal endothelialantibody(aCEN-Ab).Methods Porcinecornealendotheliawereobtainedwiththeepitheliaservingas control,CEN-Agandcornealepithelialantigen(CEP-Ag)wereisolatedandpurifiedby ion-exchangechromatographyandaffinity chromatographywith2B4.14.1monoclonalantibody.TherabbitswereimmunizedwiththeresultantCEN-AgandCEP-Ag andtheirseracollectedto harvestaCEN-Abandanti-cornealepithelialantibody(aCEP-Ab)by affinitychromatography.Results and Conclusion The relativemolecularmassof CEN-Agand CEP-Agwere43000and53000respectivelyas determinedby SDS-PAGE,withtheirimmunoglobulinsbeingIgG 2 andIgG 3 .
出处
《第一军医大学学报》
CSCD
北大核心
2002年第1期76-78,共3页
Journal of First Military Medical University
基金
第一军医大学校长基金(1994)
关键词
角膜内皮
抗原
抗体
层析
角膜移植
cornealendothelium
antigen
antibody
chromatography
