摘要
目的 探讨细胞外基质对淋巴管新生的作用。 方法 在Ⅰ型胶原蛋白凝胶中和Matrigel基膜基质凝胶上培养狗胸导管内皮细胞 ,相差倒置显微镜下观察内皮细胞形成的管状结构的形态 ,透射电镜下观察淋巴管样结构 ,用图像分析仪对管状结构作定量分析。 结果 淋巴管内皮细胞在Ⅰ型胶原蛋白凝胶上生长成单层 ,但未见管状结构形成。在细胞上面覆盖上层凝胶后 ,内皮细胞发生形态变化 ,形成管状结构。肝素组的管状结构比对照组多 ,而肝素加bFGF组的管状结构比肝素组和bFGF组多。在Matrigel基膜基质凝胶上培养的内皮细胞向凝胶上层迁移 ,形成管状结构。管状结构较细 ,细胞较长。管状结构呈明显的牵拉状态。透射电镜下 ,淋巴管内皮细胞形成的管状结构具有毛细淋巴管的形态学特征。 结论 狗胸导管内皮细胞在Ⅰ型胶原蛋白凝胶和Matrigel基膜基质凝胶中形成毛细淋巴管样结构。细胞外基质对于淋巴管内皮细胞的形态变化、细胞迁移和淋巴管新生具有促进作用。
Objective In order to investigate effects of extracellular matrix on lymphangiogenesis. Methods Endothelial cells of canine thoracic duct were incubated in type I collagen gel and on Matrigel basement membrane matrix gel.Morphologic changes of endothelial cells and tube formation were observed under phase contrast microscope,structures of lymphaticlike channels were observed with transmission electron microscope,tube formation was quantified by measuring the length and area of tubes with image analyzer. Results Lymphatic endothelial cells on type Ⅰ collagen gel grew to confluent monolayers,no tubes were found.The cells underwent morphologic changes and reorganized into lymphaticlike tubes when the cells were covered with the top gel layer.Tubes of heparin\|treated dishes were more than that of the control dishes,tubes of dishes treated with heparin and bFGF together were more than that of dishes treated with heparin or bFGF alone.Endothelial cells on Matrigel basement membrane matrix gel migrated into the upper part of the gel and formed tubes.The tubes were thin,the cells became long.The tubes were in traction obviously.In ultrathin sections,tubes formed by lymphatic endothelial cells represented morphologic characteristics of lymphatic capillary.The lymphaticlike tubes contained matrix.Conclusion\ Endothelial cells of canine thoracic duct reorganized into lymphaticlike channels in type Ⅰ collagen gel and Matrigel basement membrane matrix gel.Extracellular matrix plays important roles in morphological changes and migration of lymphatic endothelial clells and lymphangiogenesis.\;[
出处
《解剖学报》
CAS
CSCD
北大核心
2002年第1期68-73,共6页
Acta Anatomica Sinica
基金
教育部留学回国人员科研启动基金资助项目(98679)
关键词
内皮细胞
淋巴管
细胞外基质
淋巴管新生
Endothelial cell
Lymphatic vessel
Extracellular matrix
Lymphangiogenesis
