摘要
目的 探讨PKC不同亚型在原代培养脊髓神经元突起生长中的作用。 方法 采用免疫组织化学技术研究蛋白激酶C(PKC) 4种亚型 (α、βⅠ、βⅡ和ε)在胎鼠脊髓原代培养神经元突起生长过程中的表达与分布 ,借助计算机图像分析系统同步测定突起长度和胞体面积。 结果 原代培养神经元生长过程中 ,7d前随着PKC 4种亚型的表达逐渐增加 ,神经元胞体逐渐长大及突起逐渐延长 ;7d以后无论在胞体还是突起中 ,PKC 4种亚型的表达均下降 ,突起开始萎缩 ,胞体内出现空泡。相关分析发现 βⅡ亚型与突起生长关系最密切 (r =0 73,P <0 0 1)。 结论 PKC可能参与了神经元的生长过程 ;
Objective To study the role of different subtypes of PKC in the neurite outgrowth of primary cultured neurons from embryonic rat spinal cord. Methods We observed the expression and distribution of protein kinase C(α、βⅠ、βⅡ、ε subtypes) by using immunohistrochemical technique in the neurite outgrowth of the primary cultured neurons from the embryonic rat spinal cord by means of computer image analysis system to measure the lengths of neurites and the areas of bodies. Results The areas of the cultured neuronal bodies increased and the neurites prolonged while the expression of the four subtypes of PKC gradually increased before 7div(day in vitro )during primary cultured neurons growth. The expression both in bodies and neurites of neurons decreased after 7div and the processes gradually shrinked and at the same time bubbles appeared inside the bodies. Relative assay demonstrated that βⅡ subtype was most relative to the outgrowth of neurites( r =0 73, P <0 01). Conclusion\ The findings suggest that PKC involvs in the growth of neurons and βⅡ subtype of PKC plays a more important role in the neurites outgrowth of neurons.\;[
出处
《解剖学报》
CAS
CSCD
北大核心
2002年第1期38-41,共4页
Acta Anatomica Sinica
基金
国家自然科学基金资助项目 (3 95 70 3 73 )
关键词
蛋白激酶C
神经元
组织培养
Protein kinase C
Neurons
Tissue culture
Rat
