摘要
目的 探讨“延髓内脏带 (MVZ)至下丘脑室旁核 (PVN)”的儿茶酚胺能通路在“免疫 脑通讯”中的作用。 方法 应用WGA HRP逆行追踪与抗Fos和抗酪氨酸羟化酶 (TH)抗体双重免疫组织化学相结合的三重标记方法 ,即先将WGA HRP注入大鼠一侧PVN内 ,存活 4 8h后经腹腔注射免疫刺激剂脂多糖 (LPS)诱发免疫反应后 ,观察MVZ中WGA HRP逆标细胞、Fos蛋白和儿茶酚胺能神经元 (以TH为标记物 )的分布及表达状况。 结果 在MVZ内发现 7种阳性神经元 ,即HRP、Fos、TH单标细胞、Fos HRP、Fos TH、HRP TH双标细胞和Fos HRP TH三标细胞。Fos HRP双标记和Fos HRP TH三标记细胞分别占总HRP逆行标记细胞的 12 5 %和 39 6 %。 结论 MVZ对LPS免疫刺激起反应 ,并可能将免疫信息经儿茶酚胺能神经元上传至PVN ;MVZ可能作为“免疫 脑通讯”的一个中继站 ,通过“MVZ→PVN”通路起免疫调节作用。
Objective To test the possibility that the catecholaminergic projectional pathway from the medullary visceral zone(MVZ) to the paraventricular nucleus of hypothalamus(PVN) was involved in the immune\|to\|brain communication. Methods A triple labeling method in which WGA\|HRP retrogradly tracing combined with anti\|Fos and \|TH immunohistochemical staining was used.WGA\|HRP was stereotaxically injected into unilateral PVN in the rat,after a survival of 48h,animals received intraperitoneal injection of lipopolysaccharide(LPS).The distributions of the HRP retrogradely labeled neurons,Fos reactive protein and catecholaminergic neurons(tyrosine hydroxylase as marker) in the MVZ were observed. Results Under light microscope,seven types of positively stained neurons could be found within the MVZ:neurons single\|labeled with Fos,HRP or TH;neurons double\|labeled with Fos/TH,Fos/HRP or HRP/TH;and neurons triple\|labeled with Fos,HRP and TH staining.Fos/HRP double\|labeled and Fos/HRP/TH triple\|labeled neurons respectively accounted for 12\^5% and 39\^6% of total HRP\|retrogradely labeled neurons.Conclusion\ The results suggested that the MVZ could make respond to peripheral immune stimulation induced by LPS and convey the immune signals to the PVN via catecholaminergic neurons.MVZ might be a relay station in the immune\|to\|brain communication and plays a role in the neuroimmunomodulation via MVZ\|PVN pathway.\;[
出处
《解剖学报》
CAS
CSCD
北大核心
2002年第1期16-20,共5页
Acta Anatomica Sinica
基金
国家自然科学基金资助项目 (3 9770 2 5 1)
关键词
延髓内脏带
神经免疫调节
下丘脑室旁核
免疫组织化学
Medullary visceral zone
Neuroimmunomodulation
Hypothalamic paraventricualr nucleus
Immunohistochemistry
Rat
