摘要
筛选出一株产菊粉酶酶源菌株AF1 0 ,鉴定为黑曲霉 (Aspergillusniger)。PCR扩增AF1 0的内切菊粉酶基因inuA1并进行核苷酸序列分析。结果表明inuA1全长 1 5 5 1bp ,没有内含子 ,所编码的氨基酸序列中 ,存在 4个潜在的N 糖基化位点 ,其中存在菊粉酶的保守序列WMNEPN。以pUC1 1 8为克隆载体 ,以E .coliJM1 0 9为受体菌株 。
A wild-type strain AF10 producing inulinase was screened from soil samples, the strain is identified to be a A.niger. A endoinulinase gene inuA1 from A.niger AF10 was wequenced and analyzed, after PCR amplification. The results shows inuA1 is 1551 bp in length without any intron and contains 4 potential N-glycosylation sites. The conservative sequence is WMNEPN from the N-terminus. The inuA1 was cloned to pUC118 and the recombinant vector pinuA1 was obtained and transformed into E.coli JM109. The recombinant JM109/inuA1 included inuA1 was obtained.
出处
《微生物学报》
CAS
CSCD
北大核心
2002年第3期321-325,共5页
Acta Microbiologica Sinica
