摘要
目的:建立中药人参的质量控制方法。方法:高效液相色谱法,Diamonsil^(TM)(钻石)C_(18)分析柱(150mm×4.6mm,5μm),流动相为乙腈-水(22:78),流速为1.0mL·min^(-1),检测波长为203nm,柱温40℃;抗疲劳药理试验以十全大补丸为阳性对照,小鼠灌胃给药(人参总皂苷)10d后观察其游泳持续时间的变化。结果:在本色谱条件下,11份不同人参商品完整药材中人参皂苷的HPLC图谱中可检出10个相对位置稳定的色谱峰,进而确定出7个人参皂苷的共有峰,作为其定性鉴别的指标峰;人参皂苷Rg_1、Re的回收率分别为98.6％和97.8％,RSD分别为0.15％和0.13％,人参皂苷Rg_1、Re的含量分别为0.091％～0.273％和0.033％～0.258％;人参皂苷可显著延长小鼠游泳的持续时间。结论:本文所建立的人参皂苷Rg_1;、Re含量测定及人参总皂苷的HPLC图谱鉴别方法,简便、准确,可用于人参的质量控制;药理实验研究表明人参总皂苷具有明显的抗疲劳作用,且量效关系显著。
Objective: To establish a method for controlling the quality of Panax ginseng. Methods: In the HPLC method of the paper the experimental conditions were as follows; Diamonsil?C18 column (150 mm × 4. 6 mm, 5μm) , acetonitril -water (22:78) used as mobile phase, flow rate 1. 0 mL· min-1, column tempera-ture 40℃, and detection wavelength 203 nm. The antifatigue pharmacodynamic experiment was carried out as fol-lows; Shiquandabuwan served as the positive contrast, the change of the swimming time was observed after taking the medicine 10 days for mouse by ig. Results: 11 samples of Panax ginseng from different districts of China were qualitatively analyzed by HPLC. Under the chromatographic conditions, 10 relative stable peaks were detected, and 7 common peaks were definited as the qualitative marker of ginsenosides. The recoveries of the quantitative a-nalysis were 98. 6% with RSD of 0. 15% for ginsenoside Rg, and 97. 8% with 0. 13% for ginsenoside Re re-spectively. The contents of ginsenoside Rg1 and ginsenoside Re in Panax ginseng were 0. 091% -0. 273% and 0. 033%-0. 258% respectively. Ginsenosides can notably lengthen the swimming time of mouse. Conclusion: The method was simple, accurate, and can be used for the quality control of Panax ginseng. Pharmacodynamics research indicates that the ginsenosides of Panax ginseng make obvious antifatigue effect, and the dose - effect re-lation is notable.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2002年第3期173-178,共6页
Chinese Journal of Pharmaceutical Analysis
基金
国家自然科学基金(批准号:20075020)
