摘要
目的 探讨风湿骨痛药酒药槌外治法防治关节软骨退变的作用机理。方法 选用日本大耳白兔18只,随机将12只通过结扎右股静脉造成膝关节骨性关节炎模型。未造模兔6只为正常组,12只造模兔随机分为造模组和治疗组。造模8周后,治疗组施以风湿骨痛药酒药槌外治法进行治疗。造模11周后,观察软骨病理组织形态学、滑膜中NO浓度、软骨中PA及PAI活性和关节液中HA含量变化。结果 治疗组滑膜中NO浓度、软骨中PA及PAI活性和关节液中HA含量与正常组相比有显著性差异。造模组所测各项指标与治疗组相比有显著性差异。治疗组滑膜中NO浓度及软骨中PA含量明显低于造模组,而软骨中PAI含量及关节液中HA含量明显高于造模组。治疗组关节软骨退变不明显,类似正常组,而造模组关节软骨退行性变明显。结论 风湿骨痛药酒药槌外治法能通过改善骨内及周围组织的微循环,抑制滑膜组织中NO的过度产生,降低软骨中PA的活性,提高PAI的活性,消除导致关节软骨退变的内在因素,提高关节液中HA的含量,从而达到保护关节软骨、抗关节软骨退变的目的。
ve To study the mechanism of external treatment method with medicated mallet full of Fengshigutong(Chinese drugs) medicated wine for preventing and curing articular cartilage degeneration Methods 18 Japanese big-ear rabbits were divided randomly into three groups; normal group, model group and treatment group. The models of rabbits knee of osteoarthritis(OA)were established by ligating the right femoral veins of the model group and treatment group. At 8 weeks after modeling, the rabbits in the treatment group were treated with medicated mallet (full of Fengshigutong medicated wine) knocking at right knee of rabbit till the skin was red and warm. This treatment lasted for 3 weeks,once daily and 20 minutes for each time. At 11 weeks, the samples of three groups were taken to measure: (1) content of hyaluronate(HA) in syn-ovial fluid in right knee of rabbits; (2)activity of plasming open activator(PA)and inhibitor of PA(PAI)in car-tilage; (3) concentrations of nitrogen monoxide(NO)in synovium; (4)at the same time,articular cartilage of in-ternal condyle of right femur were taken to observe pathologic changes. Results (1)The HA content in syn-ovia! fluid in the treatment group was higher than that of model group,and there was significant difference(P <0.05)between the two groups. But there was no difference(P>0.05)between the treatment group and the normal group. (2)There was no difference(P>0.05)in levels of PA and PAI in cartilage and the normal group compared with the model group, the PA activity in cartilage of the treatment group increased, while the PAI activity decreased.There was significant difference(P< 0.05) between the two groups. (3)There was no difference(P>0.05)in the NO concentration in synovial tissue between the treatment group and the normal group,but there was significant difference(P<0.01)between the treatment group and the model group. (4) Pathologic changes:significant degeneration was found in articular cartilage of the model group,similar to the normal group. It was seldom found in the treatment group. Conclusion The results showed that this Chinese drugs was effective in protecting articular cartilage from degeneration, prophylaxis and treatment OA by im-proving microcirculation of articular cartilage and its surrounding tissues, inhibiting excessive generation of NO in synovial tissue to decrease liberating and diffusion of inflammatory substance,lightening PA activity in carti-lage,elevating PAI activity to eliminate the internal factor that resulted in articular cartilage degeneration,and increasing HA contents in synovial fluid.
出处
《中国骨伤》
CAS
2002年第4期220-222,共3页
China Journal of Orthopaedics and Traumatology
