摘要
为探讨穹隆海马伞损伤鼠学习记忆能力与海马胶质纤维酸性蛋白阳性细胞之间的关系 ,切断 SD成年大鼠左侧穹窿海马伞 ,用 Y迷宫和免疫组织化学结合图像分析系统测试大鼠学习记忆能力和海马胶质纤维酸性蛋白阳性细胞的变化状况及它们的相互关系。结果显示 :损伤 2周后 ,损伤组损伤侧海马 CA1 区辐射层和齿状回分子层胶质纤维酸性蛋白阳性细胞的数密度较正常组分别增多 3 0 .2 9%和 3 0 .15 % (都为 P<0 .0 1) ,胞体面积分别增加 16.0 4%和 19.42 % (都为 P<0 .0 1) ,齿状回分子层胶质纤维酸性蛋白阳性细胞体密度增大 19.40 % (P<0 .0 5 )。经相关分析 ,大鼠学习记忆能力与海马 CA1 区胶质纤维酸性蛋白阳性细胞数密度呈负相关 (r=-0 .83 6,P<0 .0 1) ,与齿状回数密度呈负相关 (r=-0 .792 ,P<0 .0 1)。
The study was intended to analyze the relationship between GFAP positive cells of hippocampus and learning and memory in rats with FF transection. The FF fiber tract was unilaterally transected in adult rats by a stereotactic knife cut. Y maze procedure and immunohistochemistry method were used to asses quantitatively the changes of ability of learning and memory and GFAP positive cells of hippocampus. At 2 weeks after lesion, There was significant increase in Nv and average area of astroglia in stratum radiatum within the CA1 region of hippocampus in lesioned groups, increased by30.29% and 16.04% respectively,but no significant increase in Vv and VvcB. Nv, VvcB and average area of astroglia of the molecular layer of dentate gyrus was increased significantly in comparison with that of the normal, increased by 30.15%,19.40% and 19.42% respectively. The Nv of GFAP positive astrocytes in CA 1 and the dentate gyrus of the lesion rat correlated with their ability of learning in place discrimination as assessed(CA 1, r= 0.836, P<0.01; The dentate gyrus, r= 0.792, P<0.01). The findings indicated that GFAP positive cells of hippocampus may be involved in learning and memory in rats with FF transection.
出处
《神经解剖学杂志》
CAS
CSCD
北大核心
2002年第1期63-66,W015,共5页
Chinese Journal of Neuroanatomy
基金
广东省重点科技项目 (2 0 0 0 2 614 0 )资助项目
