摘要
目的 :探讨聚合酶链反应对结核病诊断的临床价值。方法 :采用聚合酶链反应检测痰、纤维支气管镜抽吸物、胸水和血液标本中结核杆菌DNA。结果 :聚合酶链反应检测法的敏感性为 4 0 1% ,特异性为 92 9% ,总的阳性预测值、阴性预测值和诊断效率分别为 87 2 %、6 0 78%和 6 7% ;不同标本敏感性有较大的差异。结论 :聚合酶链反应不失为结核病快速诊断的方法 ,但其敏感性、特异性尚不能完全满足临床的需要 。
Objective:To study the diagnostic value of polymerase chain reaction(PCR) in tuberculosis.Methods:Mycobacterium tuberculosis DNA in 200 specimens,including sputum,bronchial lavage fluid,pleural effusion and blood were detected by PCR technique.Results:The sensitivity and specificity of PCR were 40 1% and 92 9%,respectively.Total positive predictive value,negative predictive value and diagnosis efficiency were 87 2%?60 78% and 67%,respectively.Conclusion:PCR technique may be useful on diagnosis of tuberculosis,but at present the sensitivity and specificity are too low to meet clinical need.
出处
《广西医学》
CAS
2002年第5期617-619,共3页
Guangxi Medical Journal
关键词
结核病诊断
聚合酶链反应
临床价值
Tuberculosis
Diagnosis,Polymerase chain reavtion(PCR)
