摘要
为研究巨噬细胞表面是否存在特异性氧化型低密度脂蛋白受体 ,选用A型清道夫受体基因敲除小鼠来进行研究。竞争抑制实验发现 ,在乙酰化低密度脂蛋白存在 (终浓度为 2 0 0mg L)的情况下 ,A型清道夫受体基因敲除小鼠的腹腔巨噬细胞对氧化型低密度脂蛋白的摄取降低了 2 4 .8% ,还有大约 80 %的氧化型低密度脂蛋白可能通过特异性受体途径被摄取。蛋白配基印迹技术发现一种膜蛋白 ,分子量为 84～ 97kDa,它不结合乙酰化低密度脂蛋白 ,只结合氧化型低密度脂蛋白 ,并且未标记1 2 5I的氧化型低密度脂蛋白能竞争性抑制1 2 5I标记氧化型低密度脂蛋白与该膜蛋白的结合 。
Aim To explore the existence of specific oxidized low density lipoprotein(ox LDL) receptor and assay its molecular weight. Method Cross competition studies and ligand blotting were performed for analyzing specific ox LDL receptor. Results As an effective competitor, acetyl LDL(ac LDL) could block the uptake of nonspecific ox LDL. Studies indicated that excess ac LDL(200 mg/L)could only inhibited 24.8% of the association and 77.1% of the degradation of ox LDL. Ligand blotting studies showed that 125 I ox LDL could bind to a protein of 84～97 kDa. The binding of ox LDL to this membrane protein was inhibited completely by the addition of excess unlabeled ox LDL. In contrast, 125 I ac LDL couldn't bind to the 84～97 kDa band. Conclusion Studies showed the existence of specific ox LDL receptor and the molecular weight of this receptor was 84～97 kDa.
出处
《中国动脉硬化杂志》
CAS
CSCD
2002年第2期105-108,共4页
Chinese Journal of Arteriosclerosis
基金
国家自然科学基金 (3 7870 3 2 6)
