摘要
为研究牡蛎提取物对动脉粥样硬化的抑制作用及其可能的作用机制 ,通过建立高脂食铒性鹌鹑动脉粥样硬化模型 ,观察了牡蛎提取物预防用药对血清脂质 (酶法 )、载脂蛋白 (免疫比浊法 )、血浆超氧化物歧化酶和脂过氧化物丙二醛 (微量快速法 )的影响 ,并对主动脉、冠状动脉和肝脏进行肉眼和光镜组织学检查。结果发现 ,牡蛎提取物 10g kg胃内连续给药 8周后 ,鹌鹑主动脉、冠状动脉内膜动脉粥样硬化斑块形成的程度明显减轻 ,于动脉粥样硬化造型 4周时测定血浆总胆固醇、甘油三酯、低密度脂蛋白胆固醇和载脂蛋白B分别为 17.4 7± 0 .78、1.16± 0 .35、10 .94± 3.17mmol L和 2 .4 7± 0 .78g L ,均较模型组明显减少 (P <0 .0 5 ,P <0 .0 1)。于动脉粥样硬化造型 8周后血浆总胆固醇、甘油三酯、低密度脂蛋白胆固醇和载脂蛋白B水平仍明显低于模型组 (P <0 .0 5 ,P <0 .0 1) ,心肌及主动脉壁中的总胆固醇和甘油三酯含量显著降低 ,血清中超氧化物歧化酶升高。结果表明 ,牡蛎提取物具有调血脂、抗动脉粥样硬化作用 。
Aim To study the inhibitory action of Oyster Extract (OE) on atherosderosis and it's mechanism. Methods The model of experimental atherosclerosis in quail was established by hyper lipoidal feed. The content of serum lipid and apolipoprotein was detected with enzyme method and immunization photoextinction. The atherosclerotic plaque lesion of aortic and coronary artery were observed by eyes and through light microscope.The serum superoxide dismutase (SOD) and malondiadehyde (MDA) in quail were observed with microcontent fast detecting method. Results Oral administration OE 10 g/(kg·d) for an 8 weeks period significantly reduced the degree of atherosclerotic lesion in aortic and coronary arterious endothelium in OE group. The serum total cholesterol (TC), triglycerides (TG), low density lipoprotein cholesterol (LDLC) and apolipoprotein B (Apo B) are 17.47±0.78, 1.16±0.35, 10.94±3.17 mmol/L and 2.47±0.78 g/L respectively in OE group at 4 weeks,and were less than model group (P<0.05,P<0.01). At 8 weeks OE marked reduced the TC, TG, LDLC, and ApoB in serum and TC, TG in cardial muscle and aorta (P<0.05,P<0.01). At the same time and serum SOD in serum was 415.55±72.48 k u/L in OE group.It was higher than model group (P<0.05). Conclusions OE has an inhibitory effect on the development of experimental atherosclerosis lesions in quails, the mechanism may be related to regulation of lipid metabilism and antioxidation action.
出处
《中国动脉硬化杂志》
CAS
CSCD
2002年第2期97-100,共4页
Chinese Journal of Arteriosclerosis
基金
国家自然科学基金 (3 92 70 3 15 )
