摘要
8周龄健康肉鸡60只随机分成2组,试验组(45只)经关节腔注射鸡源致病性金黄色葡萄球菌2.5×109cfu,复制鸡葡萄球菌性关节炎的病理模型,对照组(15只)经关节腔注射PBS。对所建立的病理模型进行临床症状观察、外周血内细菌、白细胞及其分类计数以及抗体水平的动态研究。结果显示:试验组接种后均发展为典型的关节炎症状。整个试验期内试验组的死亡率为51.11％,对照组表现正常。接种后1-5 d外周血内金黄色葡萄球菌迅速升高。试验组鸡接种后血液中白细胞(WBC)总数升高。试验组和对照组的WBC总数的差异水平在第1、5、7、21、28 d极显著(P<0.01)。异嗜性细胞和淋巴细胞总数在接种后1周内明显升高,与对照组相比差异水平极显著(P<0.01);单核细胞在第3 d上升至11.67％,显著高于对照组。淋巴细胞的转化率在接种后第3、5、7 d的转化率极显著的高于对照组(P<0.01),第7 d达到峰值70.30％。试验组的抗体水平在整个试验期内呈缓慢增长趋势。
In a chicken model of Staphylococcus aureus arthritis, clinical signs, the kinetics of he-matology and serum antibody were studied. 60 8-week healthy boilers were divided into two groups, 45 broilers in experimental group and 15 broilers in control group . Every broiler in experimental group was infected with 2. 5 × 109 cfu Staphylococcus aureus in arthral cave to stimulate staphylococcal arthritis, and control group was processed by PBS . Broilers after inoculation of bacteria(IB) were all developed into typical arthritis, with 51. 11% death rate. All controls remained normal throughout the test. After IB, white blood cell (WBC) developed significantly on the 1st day and persisted through 14 days. The a-mount of lymphocyte and heterophil counts was very significantly higher than control group (F<0. 01) within one week. Mean monocyte peaked 11. 67% on 3rd day. Eosinocyte counts reduced and basocyte counts were erratic. The rate of lymphocyte transformation within 7 days after IB was also higher than control group (P<0. 01). Serum conglomerating antibody levels were slowly risen throughout the test.
出处
《华中农业大学学报》
CAS
CSCD
北大核心
2002年第2期126-129,共4页
Journal of Huazhong Agricultural University
关键词
人共感染
葡萄球菌
鸡
血液指标
chicken, Staphylococcus aureus, arthritis, WBC, antibody
