摘要
取普拉索芦菩嫩茎作外植体，培养于附加不同种类和激素浓度的ＭＳ或改良的ＭＳ培养基上，在附加 ６－ＢＡ１．５ ｍｇ／Ｌ时，丛生芽诱导效果最佳，萌发数量最多；在附加 ６－ＢＡ１．０ｍｇ／Ｌ＋ＮＡＡ０．３ｍｇ／Ｌ时，丛生芽生长最好，芽长且健壮，；在附加Ｉ ＡＡ０．２ ｍｇ／Ｌ时，生根效果最佳；当苗高 ３ ｃｍ以上，根长达２ ｃｍ以上时，开盖 ３ ｄ移入温室（１０～１６℃），炼苗 ３ ｄ后，移栽于蛙石；河沙；腐质土为０．５：１：１．５的混合基质的土壤中，成活率达９１．３％。
Take young stems of Aloe vera var chinensis as explant ,breed additional various kinds and hormone MS of concentration or based on improved MS with culture medium while adding 6- BA 1. 5mg/L, the effect of inducement of overgrown bud is optimum and sprout quantity is the most; growth of overgrown bud is the best and bud is length and also healthy while adding 6- BA 1. 0mg/L + NAA 0.3 mg/L; when seedlings height are above 3 cm and the root length reaches over 3 cm, the effect of root develops well while adding NAA 0.2 mg/L; open lid for 3 days and then move it into greenhouse (10- 16 °C), and train seedlings after 3 days and then transplant them into vermiculite; sandy bed; humus soil with mixture medium soil by 0. 5:1:1.5, and survival rate reaches 91.3%.
出处
《湖北林业科技》
2002年第1期4-6,共3页
Hubei Forestry Science and Technology
