摘要
分别用ANS、DPH及16 -NS三种不同的标记物标记小麦黄化苗的线粒体 ,研究氯化胆碱 (cholinechloride,CC)对线粒体膜的荧光光谱、平均微粘度 (η )及ESR图谱的影响。结果表明 ,0.21 -1.79mmol·L-1 的CC均能显著降低线粒体膜的荧光强度、η 值及ESR图谱的序参数 (S)和旋转相关时间 (τc) ,表明CC可增加线粒体膜的流动性。
Effects of choline chloride (CC) on fluorescence intensity, micro-viscosity () and ESR spectra of mitochondria from wheat etiolated seedlings were studied with the fluorescence labels, ANS and DPH and a spin label, 16NS. Results indicated that the concentration of CC from 0.21 to 1.79 mmol·L-1 decreased fluorescence intensity, micro-viscosity (), sequence parameter (S) and rotating correlative time (τc) of mitochondria from etiolated wheat seedlings. CC could therefore increase the fluidity of mitochondrial membrane. All these results suggested that CC could affect the biophysical characteristics and functions of mitochondria, which shed some light on the mechanism of increased resistances against stresses in plants by CC.
出处
《生物物理学报》
CAS
CSCD
北大核心
2002年第1期19-22,共4页
Acta Biophysica Sinica
基金
863计划资助项目 (863 -819 -07 -07)
关键词
氯化胆碱
小麦
线粒体膜
膜流动性
Choline chloride
Wheat
Mitochondria
Membrane fluidity
