摘要
目的 探讨白细胞介素 1 2 (IL 1 2 )对结核病患者外周血单个核细胞 (PBMC)分泌干扰素γ(IFN γ)、白细胞介素 4(IL 4)的影响。方法 随机将 2 5例结核患者和 1 5名健康人PBMC分为RPMI1 640组、PPD组、PPD +rhIL 1 2组、PPD +anti IL 1 2组 ,采用ELISA法检测各组培养上清液中IFN γ、IL 4的水平。结果 与PPD组相比 ,加入rhIL 1 2能有效增加结核患者及健康人PBMC分泌IFN γ(分别为32 1 6± 87 7至 452 5± 1 1 1 4,387 0± 70 8至 51 5 4± 44 1 ) ,减少IL 4的分泌 (54 6± 1 1 0至 41 3±1 3 5 ,55 1± 9 5至 38 2± 1 2 7) ;而加入anti IL 1 2可抑制IFN γ的分泌 ,促进IL 4合成 ,且结核患者各组IFN γ水平均低于健康人各对应组 ,而IL 4水平无差异。复治组IFN γ、IL 4水平均低于初治组 (P<0 0 5)。结论 IL 1 2通过诱导IFN γ分泌 ,抑制IL 4合成 ,从而调节TH1 /TH2 平衡 ,对结核分支杆菌感染患者产生保护性的免疫反应。IL 1 2可作为结核免疫调节剂 。
Objective To investigate the effect of IL 12 on the production of IFN γ and IL 4 by peripheral blood mononuclear cells(PBMC) from patients with tuberculosis Methods PBMCs were isolated and cultured with RPMI 1640, PPD, PPD+rhIL 12, PPD+anti IL 12, respectively The level of IFN γ and IL 4 was detected with ELISA Results Compared with the PPD group, IL 12 increased the production of IFN γ (321 6±87 7 vs 452 5±111 4, 387 0±70 8 vs 515 4±44 1, respectively), but decreased the production of IL 4 (54 6±11 0 vs 41 3±13 5,55 1±9 5 vs 38 2±12 9,respectively) in the patients and the healthy individuals; anti IL 12 blocked this effect The level of IFN γ was lower in tuberculosis patients than that in the healthy controls The IL 4 level was not different between the two groups( P >0 05) The levels of IFN γ and IL 4 were lower in recurrent tuberculosis than those in the newly diagnosed cases ( P <0 05) Conclusions IL 12 induced IFN γ production and inhabited IL 4 expression, modulating the balance between TH 1 and TH 2 cytokines, suggesting that IL 12 enhances the host defense against tuberculous infection IL 12 may be used for treatment of tuberculosis and vaccine development
出处
《中华结核和呼吸杂志》
CAS
CSCD
北大核心
2002年第5期292-295,共4页
Chinese Journal of Tuberculosis and Respiratory Diseases
