摘要
目的 探讨吸烟时转化生长因子 β1 (TGF β1 )在吸烟引起的慢性支气管炎与肺气肿中的作用。方法 建立吸烟时引起的慢性支气管炎与肺气肿的动物模型 ,2 0只雄性叙利亚金黄地鼠分为正常组和吸烟组 ,每组各 1 0只 ;体外培养支气管上皮细胞并经香烟烟雾提取物 (CSE)刺激。观察肺内和支气管上皮细胞TGF β1 mRNA和蛋白表达情况。结果 吸烟组于 3个月时出现慢性支气管炎和肺气肿的病理改变 ,免疫组化结果显示 ,吸烟组TGF β1 的阳性强度 (2 .75± 0 2 3)与正常组 (0 .84± 0 39)比较差异有显著性 (P =0 .0 0 1 )。两组的TGF β1 mRNA吸光度分别为 1 .2 8和 0 .98。支气管上皮在CSE刺激后 ,细胞形态发生明显变化 ,TGF β1 的表达增强 ,与单纯培养组比较差异有显著性 (2 .67± 0 1 6vs0 .85± 0 54 ,P =0 .0 0 1 )。结论 烟雾刺激造成气道上皮的损伤 ,产生大量的TGF β1 。
Objective To study the role of transforming growth factor β 1(TGF β 1) in the pathogenesis of chronic bronchitis and emphysema. Methods An animal model of chronic bronchitis and emphysema was developed in hamsters by chronic smoke inhalation. The expression of TGF β 1 mRNA and protein in the pulmonary tissue was observed. The bronchial epithelia was stimulated with cigarette smoke extract (CSE) in vitro and the expression of TGF β 1 was measured.Results 3 months after smoking, the animals developed chronic bronchitis and emphysema. TGF β 1 immunoreactivity in the pulmonary tissue and cultured bronchial epithelia increased significantly as compared to the normal control (2.75±0 23 vs 0.84±0 39, P =0.001 and 2.67±0 16 vs 0 85±0 54, P =0.001). The expression of TGF β 1 mRNA was also increased in the animal model (1.28 vs 0.98). Conclusions Smoking can induce over expression of TGF β 1 in bronchial epithelia, which may be one of the mechanisms for smoking induced chronic bronchitis and emphysema.
出处
《中华结核和呼吸杂志》
CAS
CSCD
北大核心
2002年第5期284-286,共3页
Chinese Journal of Tuberculosis and Respiratory Diseases
基金
国家科委"九五"攻关课题资助项目 ( 96 90 6 0 2 16 )
