摘要
利用建立的一种酶联免疫吸附试验 (ELISA)对大鼠胃肠道食糜中的 β 酪啡呔 7(β Casomorphin 7)样物质进行了检测分析。动物试验采用30只成年大鼠 ,随机分为 5组。对照组Ⅰ ,仅喂葡萄糖盐水 ;对照组Ⅱ ,喂大豆和饮水 ;试验组Ⅰ ,仅喂牛奶 ;试验组Ⅱ ,喂不含乳的普通饲料和饮水 ;试验组Ⅲ ,喂混有乳的普通饲料和牛奶。各组均自由采食 ,4d后处死。采集胃和十二指肠食糜 ,对其中 β Casomorphin 7样物质进行定量分析。结果 :在试验组Ⅰ和Ⅲ大鼠的胃、十二指肠食糜中检测到显著量的 β Casomorphin 7样物质 ,平均含量分别为 (1 60 52± 1 0 1 9) ,(1 0 6± 6 85)ng/g和 (2 2 0 48± 1 1 91 ) ,(1 4 2 84±7 72 )ng/g。试验组Ⅱ大鼠的胃和十二指肠食糜中也检测到 β Casomorphin 7样物质 。
Casomorphin 7 like materials of digesta by ELISA method in rat′s stomach and duodenum.Thirty mature rats were divided randomly into five groups.In control group Ⅰ,rats were fed with glucose saline;In control group Ⅱ,with water and soybean;In experiment group Ⅰ,rats were only fed with milk;In experiment group Ⅱ,with water and general rat feed.In experiment group Ⅲ,with milk and milk powder mixed general rat feed(W∶W=1∶1).Rats were continuously fed four days.There were no β Casomorphin 7 like materials detected in stomach and duodenum′s digesta of two control groups;Of experiment group Ⅰ and Ⅲ,remarkable concentration of β Casomorphin 7 like materials were detected.Average concentration was(160 52±10 19),(106±6 85)ng/g digesta and (220 48±11 91),(142 84±7 72)ng/g digesta;In experiment group Ⅱ,there were considerable amount of β Casomorphin 7 like materials detected.
出处
《畜牧与兽医》
北大核心
2002年第1期8-9,共2页
Animal Husbandry & Veterinary Medicine
基金
国家自然科学基金资助 (30 0 70 565)
