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ELISPOT技术在抗肿瘤疫苗研究中的应用 被引量：2

The Application of ELISPOT on the Research of Anti-Tumor Vaccine
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摘要 在肿瘤疫苗治疗前后 ,如何准确的定量、定性判定肿瘤抗原特异性T细胞反应 ,评价疗效 ,是影响抗肿瘤疫苗发展的关键。ELISPOT法是近年来逐渐建立起来的一种通过检测细胞因子评价T淋巴细胞功能和特异性的新方法。它能够对T淋巴细胞直接进行检测 ,不需要体外扩增 ,特异性、敏感性都有所提高。经过可行性分析和多中心对比研究 ,该技术不仅可用于监测肿瘤疫苗的疗效 ,也能用来鉴定肿瘤抗原表位。很有可能在完成的与其他方法的比较后ELISPOT将会成为定量分析肿瘤或病毒特异性的T细胞反应的首选。
作者 禄韶英 潘承恩 隋延仿
机构地区 西安交通大学第一医院普外科 第四军医大学基础部病理学教研室肿瘤抗原肽实验室
出处 《中国肿瘤生物治疗杂志》 CAS CSCD 2002年第1期72-74,共3页 Chinese Journal of Cancer Biotherapy
关键词 研究 ELISPOT技术 疫苗 肿瘤抗原 抗原表位 免疫反应
分类号 R730.51 [医药卫生—肿瘤]
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参考文献19

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同被引文献26

  • 1胡廷辉,应敏刚.热休克蛋白70多肽复合物与肿瘤免疫[J].福建医科大学学报,2006,40(3):308-310. 被引量：2
  • 2应敏刚,刘胜,龚福生,郑秋红.快速蛋白液相色谱系统分离与纯化小鼠结肠癌细胞热休克蛋白70[J].中华肿瘤防治杂志,2007,14(18):1386-1388. 被引量：2
  • 3Mazzaferro V,Coppa J,Carrabba M G,et al.Vaccination with autologous tumor-derived heat-shock protein Gp96 after liver resection for metastatic colorectal cancer[J].Clinical Cancer Research,2003,9(9):3235-3245.
  • 4Pal'tsev M A,Severin S E,Danilevskiǐ M I,et al.Regulation of human dendrite cells' physiological functions with the recombinant heat shock protein Hsp70[J].Ross Fiziol Zh Im I M Sechenova,2009,95(10):1011-1023.
  • 5Janetzki S,Palla D,Rosenhauer V,et al.Immunization of cancer patients with autologous cancer-derived heat shock protein Gp96 preparations:a pilot study[J].Int Cancer,2000(88):232-238.
  • 6Nishikawa M,Takemoto S,Takakura Y.Heat shock protein derivatives for delivery of antigens to antigen presenting cells[J]..Int J Phar,2008,354(1-2):23-27.
  • 7Enomoto Y,Bharti A,Khaleque A A,et al.Enhanced immunogenicity of heat shock protein 70 peptide complexes from dendritic cell-tumor fusion cells[J].Immunol,2006,177(9):5946-5955.
  • 8Gong J,Zhang Y,Durfee J,et al.A heat shock protein 70-based vaccine with enhanced immunogenicity for clinical use[J].Immunol,2010,184(1):488-496.
  • 9Etminan N,Peters C,Lakbir D,et al.Heat-shock protein 70-dependent dendritic cell activation by 5-aminolevulinic acid-mediated photodynamic treatment of human glioblastoma spheroids in vitro[J].Br J Cancer,2011,105(7):961-969.
  • 10Beatrice T, Ina H, Claudia R et al. Vaccination with Mage-3A1 peptide - pulsed mature, monocyte- derived dendritic cells expands specific cytotoxic T cells and induces regression of some metastases in advanced stage IV melanoma[J] .J Exp Med, 1999;190(11) :1669- 1678.

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中国肿瘤生物治疗杂志
2002年 第1期

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