摘要
利用高效液相色谱法 ,在C18柱上以甲醇 乙腈 0 0 1mol/L草酸溶液 (pH 2 0 ) (体积比为 11∶2 2∶6 7)为流动相 ,采用 2 6 7nm紫外光进行检测 ,在 2 2min内将 7种四环素类抗生素全部洗脱并达到基线分离。探讨了流动相的pH值、草酸的浓度、流动相中有机相的比例以及检测波长等因素对分离度和灵敏度的影响。采用标准加入法定量 ,对两种实际样品进行了分析。结果表明 :该方法操作简单、灵敏度高。
A method for the determination of 7 tetracycline antibiotics (TCs) by reversed phase high performance liquid chromatography is described TCs were successfully separated on a Diamonsil TM C 18 column(250 mm×4 6 mm i d , 5 μm) using methanol acetonitrile 0 01 mol/L oxalic acid (pH 2 0)(11∶22∶67, volume ratio) as mobile phase at a flow rate of 0 8 mL/min and detected at 267 nm within 22 min Effects of the pH value of the mobile phase, concentration of the mobile phase buffer, elution composition and detection wavelength on the response and retention were studied. Tetracycline(TC) and oxytetracycline(OTC) in medicinal tablets were quantitated by standard added method It has been proven that the method is fast, accurate and suitable for routine analysis
出处
《色谱》
CAS
CSCD
北大核心
2002年第1期49-51,共3页
Chinese Journal of Chromatography
关键词
反相高效液相色谱法
测定
四环素类抗生素
紫外检测
分析
reversed phase high performance liquid chromatography
tetracycline antibiotics
UV detection
