摘要
用超临界CO2 流体及共溶剂乙醇萃取丹参中的 3种丹参酮 ,分别采用正交设计法和系统法考察了萃取中的主要影响因素 ;采用高效液相色谱法 (HPLC) ,在甲醇 水 (体积比为 80∶2 0 )溶液为流动相和检测波长为 2 80nm的条件下 ,以外标法检测了萃取产物中 3种丹参酮的含量。实验得到的最佳条件为 :萃取压力 2 0MPa ;萃取温度 4 5℃ ;分离温度 35℃ ;共溶剂 95 % (体积分数 )乙醇 ;流量 1 0mL/min。建立的HPLC测定方法简便快捷 ,准确度高 ,重现性良好 ,相关系数r为 0 9994～ 0 9998,相对标准偏差RSD为 2 37%～ 3 4 7%。
The extraction of three tanshinones from the root of Salvia miltiorrhiza Bunge by supercritical carbon dioxide (SC CO 2) fluid with ethanol as co solvent was investigated using four factor and three level orthogonal design method and the systematic method The optimized conditions were established as follows: 95% ethanol was selected as a co solvent, flow rate of co solvent was 1 0 mL/min, extraction pressure was 20 MPa, extraction temperature was 45 ℃,separation temperature was 35 ℃. The sample solution was separated on a Nova Pak C 18 column (3 9 mm i d ×300 mm,5 μm)with CH 3OH H 2O (80∶20, V/V ) as the mobile phase and detected at 280 nm The correlation coefficients were 0 999?4-0 999?8 and the relative standard deviations were 2 37%-3 47% The detection limits were 0 473 mg/L, 0 184 mg/L and 0 452 mg/L for tanshinone ⅡA, tanshinone Ⅰ and cryptotanshinone respectively
出处
《色谱》
CAS
CSCD
北大核心
2002年第1期40-42,共3页
Chinese Journal of Chromatography
关键词
丹参酮ⅡA
丹参酮Ⅰ
超临界二氧化碳
萃取
高效液相色谱法
丹参
隐丹叁酮
中药分析
supercritical carbon dioxide
extraction
high performance liquid chromatography
Salvia miltiorrhiza Bunge
tanshinone ⅡA
tanshinone Ⅰ
cryptotanshinone
