摘要
目的 :探讨去甲肾上腺素 (NE)在缺血性室颤及缺血 /再灌注性室颤发生中的作用及机制。方法 :运用高效液相色谱 电化学法测定离体豚鼠缺血性室颤心脏 (n =2 4 )及缺血 /再灌注性室颤心脏 (n =2 6 )流出液中NE的含量 ,并与未发生室颤心脏 (n =2 9)相比较 ,用全细胞膜片钳记录技术检测NE对缺氧豚鼠心肌细胞 (n =8)钙通道电流 (ICa)的影响。结果 :发生缺血性室颤心脏及缺血 /再灌注性室颤心脏NE释放量明显高于未发生室颤心脏 (P <0 0 1) ;NE可明显增加缺血及缺血 /再灌注心肌细胞ICa(P <0 0 1,P <0 0 5 )。结论 :NE增加心肌细胞ICa为其致缺血及缺血 /再灌注性室颤的机制之一。
Aim:To study the influence of norepinephrine(NE) on ischemia induced ventricular fibrillation(Vf) and reperfusion induced Vf Methods:The levels of NE in the outflow from the isolated hearts of guinea pigs with ischemia induced Vf( n =24)and ischemia reperfusion induced Vf( n =26) were determined by high performance liquid chromatography(HPLC) with electrochemical detection.The results were compared with that of the hearts without Vf( n =29). The effects of NE on calcium current( I Ca ) through isolated guinea pig cardiomyocytes( n =8) under hypoxia were detected with the whole cell patch clamp method.Results:The NE release of hearts with ischemia induced Vf and reperfusion induced Vf was markedly higher than that of hearts without Vf( P <0.01),and NE enhanced I Ca in cardiocmyoytes under both hypoxia condition and hypoxia/reperfusion condition significantly( P<0.01 and P <0.05 respectively).Conclusion:The results indicate that the enhance of I Ca resulted from NE contributes to Vf induced by ischemia and ischemia/reperfusion.
出处
《郑州大学学报（医学版）》
CAS
北大核心
2002年第2期195-197,共3页
Journal of Zhengzhou University(Medical Sciences)
