摘要
目的 探讨完全弗氏佐剂 (CFA)在预防非肥胖糖尿病 (NOD)鼠胰岛炎和糖尿病机制中的作用。方法 将 3周龄NOD雌鼠随机分入CFA组、磷酸盐缓冲液 (PBS)对照组 ,分别给予后足板一次性注射CFA 5 0 μl、PBS 5 0 μl,于 6周龄、12周龄、30周龄时观察胰岛 β细胞凋亡、胰岛炎程度和糖尿病发病率。结果 6周、12周时CFA组胰岛炎积分、β细胞凋亡率均明显低于同期PBS组 (P <0 0 5 ,P <0 0 1) ,6周龄、12周龄胰岛炎积分与 β细胞凋亡率存在正相关。到 30周龄时 ,CFA组 11只鼠只有 1只发生糖尿病 (1/11) ,明显低于PBS组 [11只中有 9只 (9/11) ],P =0 0 0 1。结论 早期给予NOD鼠CFA可明显减轻其胰岛炎和糖尿病的发生 ,其机制与抑制 β细胞凋亡有关。目的 探讨完全弗氏佐剂 (CFA)在预防非肥胖糖尿病 (NOD)鼠胰岛炎和糖尿病机制中的作用。方法 将 3周龄NOD雌鼠随机分入CFA组、磷酸盐缓冲液 (PBS)对照组 ,分别给予后足板一次性注射CFA 5 0 μl、PBS 5 0 μl,于 6周龄、12周龄、30周龄时观察胰岛 β细胞凋亡、胰岛炎程度和糖尿病发病率。结果 6周、12周时CFA组胰岛炎积分、β细胞凋亡率均明显低于同期PBS组 (P <0 0 5 ,P <0 0 1) ,6周龄、12周龄胰岛炎积分与 β细胞凋亡率存在正相关。到 30周龄时 ,CFA组 11?
Objective To investigate the role of complete Freund′s adjuvant (CFA) in preventing insulitis and diabetes in non obese diabetic(NOD) mice. Methods Three week old female NOD mice were randomly devided into CFA group and phosphate buffered solution (PBS) group. The medications were injected once into the pad of hind foot with 50 μl CFA or 50 μl PBS respectively. Mice of the two groups were sacrificed at 6, 12 and 30 weeks of age respectively. Staining and counting the apoptotic β cells in islets and evaluating insulitis severity and diabetes incidence were then carried out. The pancreatic sections were stained with hermatoxylin and eosin to score insulitis, and the apoptotic β cells in islets were confirmed with the di labeling technique of the TUNEL in situ end labeling method combined with standard sensitive avidin biotin complex (sABC) immunohistochemical method. Results The insulitis score of CFA treated mice at 6 and 12 weeks of age was significantly lower than that of PBS treated mice at the corresponding time respectively ( P 6w <0.05, P 12w <0.05). The apoptotic β cells were fewer in CFA treated mice than that in PBS treated mice at 6 and 12 weeks respectively ( P 6w <0.01, P 12w <0.01). A positive correlation was found between the apoptotic β cell rates and the insulitis scores at 6 and 12 weeks of age. The diabetes incidence in CFA treated mice was lower than that in PBS treated mice ( P =0.001). Conclusion Early treatment with CFA can diminish insulitis and prevent the development of autoimmune diabetes in NOD female mice; this may result from inhibition of apoptotic β cells.
出处
《中华内科杂志》
CAS
CSCD
北大核心
2002年第4期229-232,共4页
Chinese Journal of Internal Medicine
基金
国家自然科学基金资助 (3 9770 3 5 2 )
