摘要
引用未被取代的葡萄糖单元的序列长度分布的概念,对羧甲基淀粉醚在α-淀粉酶和葡萄糖淀粉酶的混合酶试剂作用下,发生的酶降解的准确位置进行了数学推算。结果表明酶降解只发生在两个相邻的未被取代的葡萄糖单元之间,用酶降解前后1000个葡萄糖单元的分子链数之差(△1000/(?)_n)和降解后生成的葡萄糖占未被取代的葡萄糖单元数的百分含量(GI)可表征取代基在分子链上的分布。此外还对取代度和取代基在分子链上的分布与抗酶降解性的关系进行了研究。
The concept of the sequence length distribution of unsubstituted anhydroglulose units has been cited to study the mechanism of anti-degradation of CMS in the mixed enzymic agent of α-amylase and amyloglulosidase. The result indicates that the cleavage of CMS chain occurs at random only between adjacent unsubstituted residues. The distribution of substituent groups along the chain is characterized by means of two parameters: the percentage of glulose resultant by enzymetic hydrolysis in unsubstituted glulose units(GI); the number of chain breaks off per 1000 units(Δ1000/ (?)_n). The relationship between the reistance to enzymic degradation of CMS and its degree of substitution and the distribution of substituent groups along the chain has also been studied.
出处
《天津大学学报》
EI
CAS
CSCD
1991年第4期82-87,共6页
Journal of Tianjin University(Science and Technology)
关键词
羧甲基淀粉醚
抗酶降解
淀粉
carboxymethyl starch, α-amylase, amyloglucosidase, resistance to enzymic degradation, distrfution of substituent groups along the chain
