摘要
蛋白质在微生物研究中可用作生物标记物, 故在生物技术中其表征工作很重要. 各种质谱技术已被用于细菌的表征[1～3]. 1994年, Cain等[4]首先报道了将色谱和基体辅助激光解离/电离飞行时间质谱(MALDI-TOF-MS)用于细菌的辨识. Holland等[5]采用高质量离子MALDI-TOF-MS对细菌全细胞进行辨识. 最近, Lay[6]对该技术的细菌辨识做了综述. MALDI-TOF-MS是目前对细菌全细胞分析的最佳手段.
The theoretical basis of temperature constrained cascade correlation network(TCCCN) was in-troduced briefly. The TCCCN models were used to identify various bacteria from their matrix-assistedlaser desorption/ionization time-of-flight mass spectrometric (MALDI-TOF-MS) data. The identificationof eight different bacteria and the bacteria identification based on different culture time have been dis-cussed. The results show that the TCCCN could produce robust and feature-oriented models for bacteria i-dentification based on MALDI-TOF-MS. However, mis-identification may occur for some bacteria sam-ples due to variation in cell biology under environmental conditions.
出处
《高等学校化学学报》
SCIE
EI
CAS
CSCD
北大核心
2002年第4期570-572,共3页
Chemical Journal of Chinese Universities
基金
教育部中青年骨干教师基金资助.
