摘要
细胞内胆固醇代谢的失衡和细胞凋亡都与动脉粥样硬化的发生有关 .为了研究两者之间的关系 ,我们把猪的主动脉平滑肌细胞与 15mg/L氧化低密度脂蛋白共同孵育 72h ,发现细胞内胆固醇酯与总胆固醇的比值由 2 6 2 %增加到 6 4 1% ,并且细胞内胆固醇酯的积聚有剂量依赖关系 ,表明细胞已经转化为平滑肌源性的泡沫细胞 .另外 ,使用荧光显微镜、激光共聚焦显微镜和流式细胞仪分别发现 ,与氧化低密度脂蛋白共孵育的细胞有典型的凋亡形态改变 .从实验可以推测 ,由氧化低密度脂蛋白诱导的平滑肌细胞凋亡 ,除了低密度脂蛋白氧化的因素外 ,也可能与细胞内胆固醇酯与总胆固醇的比值升高有关 .
Both cellular cholesterol metabolism imbalance and apoptosis are related to the development of atherosclerosis. To investigate the relationship between the cellular cholesterol metabolism and apoptosis, the porcine aortic smooth muscle cells were cultured with medium 199 containing 15 mg/L oxidized low density lipoprotein (Ox LDL) for 72 h , the ratio of cellular cholesteryl ester to total cholesterol increased from 26 2% to 64 1%, and Ox LDL induced accumulation of cellular cholesteryl ester in a concentration dependent manner in the cells. It indicated that the vascular smooth muscle cells had transformed to foam cells. In addition, cells incubated with oxidized low density lipoprotein had characteristic of apoptosis, as determined by fluorescence microscope, laser scanning confocal microscope and flow cytometry. From this findings, it was speculated that the induction of apoptosis may be related to the raise of the ratio of cellular cholesteryl ester to total cholesterol besides oxidation of low density lipoprotein in vascular smooth muscle cells.
出处
《生物化学与生物物理进展》
SCIE
CAS
CSCD
北大核心
2002年第2期217-222,共6页
Progress In Biochemistry and Biophysics
基金
湖南省自然科学基金资助项目 ( 97JJY2 0 2 5 )~~
关键词
主动脉
氧化低密度脂蛋白
胆固醇酯
平滑肌细胞凋亡
动脉粥样硬化
oxidized low density lipoprotein, cholesteryl ester, apoptosis, vascular smooth muscle cells, atherosclerosis
