摘要
目的:观察COX-2在急性肝损伤中的表达,探讨COX-2与急性肝损伤的关系。 方法:将50只(?)Wistar大鼠随机分为正常组10只,对照组10只腹腔注射CCL4原液1mL·kg^(-1)、实验组30只分3组腹腔注射CCL4原液1mL·kg^(-1),2h后每组分别给予20mg·kg^(-1)、40mg·kg^(-1)和80mg·kg^(-1)塞来昔布灌胃。 结果:血清学检测结果显示CCL4组和CCL4+Celecoxib组大鼠血清ALT,AST和LPS均较正常大鼠组明显增高(ALT:1392±16 vs 624±5,AST:1803±18 vs 748±6,LPS:124±61 vs 60±50 P <0.05),CCL4组与CCL4+Celecoxib组大鼠血清ALT、AST和LPS水平存在显著差异(ALT:1790±13,3301±20,4500±32 vs 1392±16,AST:2183±21,3946±30,4903±23vs 1803±18,LPS:143±37,186±77,232±35 vs 124±61,P<0.05),HE染色光镜下示CCL4组和CCL4+Celecoxib组大鼠大量肝细胞肿胀、空泡变性和脂肪变性,部分肝细胞坏死,免疫组织化学检测结果显示NF-kBP65在部分正常肝组织弱阳性表达,CCL4组与CCL4+Celecoxib组大鼠均有NF-kBP65的阳性表达,且两组NF-kBp65的表达存在显著差异(0.175±0.045,0.196±0.024,0.217±0.029 vs 0.217±0.029 P<0.05);COX-2在正常肝组织无表达,CCL4+Celecoxib组大鼠较CCL_4组大鼠COX-2表达明显增高(0.148±0.025,0.158±0.
AIM: To study the expression of cyclooxygenases-2 and its relationship with acute hepatic injury.METHODS:Fifty Wistar rats were divided into five groups: normal group 10 rats, control group 10 rats receiving ip CCL4 1mL·kg-1, and three model groups (10 rats in each group)ip CCL4 1mL·kg-1, and after two hours ig Celecoxib 20mg · kg-1, 40mg · kg-1, 80mg · kg-1 were administered, respectively.RESULTS: The levels of serum ALT, AST and LPS in the control group were higher than these in normal group (ALT: 1392±16 vs 624±5,AST: 1803± 18 vs 748±6,LPS: 124 ± 61 vs 60 ±50, P<0.05). These indices also showed a significant difference between control and model groups (ALT: 1790 ± 13, 3301 ± 20, 4500 ± 32 vs 1392 ± 16, AST: 2183 ± 21, 3946 ± 30, 4903±23 vs. 1803±18,LPS: 143 ± 37, 186 ±77,232± 35 vs 124 ±61. P<0.05). HE stains showed hepatic cell swelling, vacuolation and steatosis in control and model groups. Immunoh stochemical stain showed that expression of COX-2 and NF-kBp65 in model groups were higher than these in control group(NF-kBp65: 0.175 ± 0.045, 0.196±0.024, 0.217±0.029 vs. 0.217±0.029 P <0.05; COX-2: 0.148 ±0.025, 0.158 ±0.030, 0.169± 0. 017 vs 0.146±0.024 P<0.05).CONCLUSIONS: Hepatic injury induced the expression of COX-2 in hepatocytes which might be a protective role against injury by the organs. The levels of NF-kBP65 rose with the increase of COX-2 levels.
出处
《世界华人消化杂志》
CAS
2002年第1期24-27,共4页
World Chinese Journal of Digestology
