摘要
目的 :提取人类高相对分子质量唾液粘蛋白 (MG1)并对其性质进行初步鉴定。方法 :用十六烷基三甲基溴化铵 (CETAB)沉淀 ,羧甲基葡聚糖 (CM Sephadex)离子交换和葡聚糖凝胶 (SephadexG 2 0 0 )过滤层析从人类除腮腺外唾液中提取MG1,并对其蛋白质含量、氨基酸组成 ,纯度及相对分子质量进行检测。结果 :提取的MG1蛋白质含量为 14 .17% ,氨基酸组成分析表明苏氨酸、丝氨酸、脯氨酸、丙氨酸为优势基酸 ,占 45 .6 % ,相对分子质量为 5 0 00 0 0～ 10 0 0 0 0 0。结论 :此技术提取MG1方法简单温和 。
砄bjective:To purify and characterize human high molecular eight salivary mucin(MG1).Methods: MG1 was purified by 10%(w/v) cetyltrimethylammonium bromid precipitation, CM Sephadex ion exchange chromatography and sephadax G 200 gel filtration chromatography. The protein content was studied with Folinin Lowrys analysis and characterized by PAGE and SDS PAGE electrophoresis.Results:The data of PAGE showed that the purified glycoprotein was free of contaminating proteins;those SDS PAGE showed that the melocular weight of the glycoprotein was between Mr 500 000 and 1 000 000.Protein quantitative analysis showed that it contained 14.17% of protein.Amino acid analysis revealed that it contained 17 kinds of amino acid;Thr,Ser,Pro and ALa were the dominant amino acid(45.6% of the total).Conclusion: The data indicate the applied technique is reliable for purification MG1 from saliva.
出处
《实用口腔医学杂志》
CSCD
北大核心
2002年第2期117-119,共3页
Journal of Practical Stomatology
基金
国家自然科学基金资助项目
编号 :391 70 31 1 78
关键词
唾液
粘蛋白类
提纯
鉴定
人
Salivaiy Mucin
Purifications
Certification
