摘要
目的 研究细胞凋亡抑制基因Bcl-2和细胞凋亡促进基因Bax在肝细胞癌(HCC)中的表达情况.方法 应用免疫组织化学SABC法对35例HCC标本切片后进行Bcl-2和Bax蛋白染色,根据阳性率高低分析其表达与临床机理的关系.结果 HCC中Bcl-2和Bax阳性细胞均为胞浆染色.35例HCC中Bcl-2和Bax蛋白各7例和13例阳性.Bcl-2和Bax蛋白在小肝癌更多见.全部7例Bcl-2蛋白阳性HCC中6例为组织学分级Ⅱ级.Bax蛋白在组织学分级Ⅰ级的表达率明显低于Ⅱ+Ⅲ级.Bcl-2蛋白表达有随临床分期升高而降低的趋势.Bcl-2和Bax蛋白联合表达仅见于4例HCC.结论 Bcl-2和Bax蛋白表达与HCC的组织学分级有关.Bcl-2和Bax蛋白在预后相对较好的HCC中较高表达,提示Bcl-2和Bax可能具有评估预后的价值.
Objective: To investigate the expression of Bcl-2, the apoptosis inhibitor, and Bax, the apoptosis promoter in hepatocellular carcinomas (HCCs). Methods: 35 formalin-fixed and paraffin-embedded samples of histologically confirmed HCC, were examined using immunohis to chemical staining for Bcl-2 and Bax protein. Results : 7 of 35 HCCs were positive for Bcl-2 protein and 13 of 35 positive for Bax protein. Immunoreactivity for Bcl-2 protein was present in 24 of 35 HCC cases and Bax protein in 25 of 35 cases. Furthermore, all positive cells were cytoplasmic stained. 6 of 7 Bcl-2 protein positive HCCs were (histological grade Ⅱ ), Bax protein expression had little association with histological grading. Co-expression of Bcl-2 and Bax protein was only present in 4 cases. At any rate, a significant high Bcl-2 and Bax protein expression was detected in small HCCs. Also, Bcl-2 protein expression tended to decrease with clinical stage. Conclusion: Bcl-2 and Bax immunohistochemical expression in HCCs seems to be associated with histological grading. Also, Bcl-2 protein seems to be relative to tumors with good prognosis. It supports the hypothesis that Bcl-2 expression has prognostic value.
出处
《中国现代普通外科进展》
CAS
1999年第1期25-27,30,共4页
Chinese Journal of Current Advances in General Surgery
