摘要
目的 研究 35 .8GHz毫米波照射诱导人肝癌细胞株BEL74 0 4凋亡。方法 选取BEL74 0 4人肝癌细胞体外培养 ,随机分为 4组 ,分别为对照组、毫米波照射 30min组、氟尿嘧啶 (5 FU)组、毫米波联合 5 FU组。用荧光显微镜观察细胞核的变化 ;DNA片段化分析检测染色体断裂情况 ;用流式细胞仪检测凋亡率。结果 BEL74 0 4细胞经毫米波照射后诱发的细胞死亡具有细胞凋亡的形态学特征 ;照射 30min组和 5 FU组诱导细胞凋亡的比例高于对照组 ,分别为 (14 .33± 2 .6 6 ) % ,(18.5 8±2 .5 7) %和 (3.2 1± 1.0 6 ) % ,毫米波与 5 FU联合明显增加细胞凋亡率 ,达 (2 7.91± 3.6 6 ) %。结论 35 .8GHz毫米波照射可诱导BEL74 0 4肝癌细胞凋亡 ,与 5
Objective To study the effect of millimeter wave radiation on human hepatoma cell. Methods BEL7404 hepatoma cells were cultured in vitro and randomly divided into 4 groups: the control group, the group radiated by millimeter wave for 30 min, the group treated with Fluorouracil(5 FU), and the group radiated by millimeter wave and treated with 5 FU simultaneously at same time. The ability of 35.8 GHz millimeter wave to induce the apoptosis of hepatoma cell was evaluated by analyses of fluorescence microscopy, DNA fragmentation assay and flow cytometry assay. Results BEL7404 cells radiated by the millimeter wave had the typical characteristics of apoptosis. Comparaed with the control group [(3.21± 1.06)%], the apoptosis rates were higher in 30 min radiating or/and 5 FU groups[ (14.33± 2.66)%, (18.58± 2.57)%, (27.91± 3.66)%]. Poly adp ribose polymerase(PARP) was found to be cleavaged in all the cells in millmeter wave radiation or/and 5 FU groups. Conclusion Radiation of 35.8 GHz could induce apoptosis of BEL7404 cell in vitro, and could act synergistcally with 5 FU treatment.
出处
《中华消化杂志》
CAS
CSCD
北大核心
2002年第2期103-105,共3页
Chinese Journal of Digestion
