摘要
【摘要】背景和目的:土贝母皂苷是由传统中药土贝母块茎中分离得到的,由土贝母苷甲(79%)和土贝母苷乙(21%)组成。本研究旨在探讨土贝母皂苷抗肿瘤作用的机制。方法:MTT法检测土贝母皂苷对肿瘤细胞生长的抑制作用,流式细胞仪分析细胞周期,荧光显微镜和电子显微镜观察细胞形态,琼脂糖凝胶电泳检测DNA电泳图谱的变化。结果:土贝母皂苷对人宫颈癌HeLa细胞的生长具有强抑制作用,且这种作用呈时间剂量依赖关系,处理24、48和72h的IC50值分别为20.0、18.8和8.8μmol/L。流式细胞术分析显示,15、30、35μmol/L土贝母皂苷处理5h,HeLa细胞G2/M期细胞数从9.80%分别增加到21.90%、25.92%和27.00%;处理12h,G2/M期细胞数增加更显著,从8.20%分别增加到21.40%、31.15%和34.55%。40μmol/L土贝母皂苷处理一定时间,形态学检查发现细胞核明显皱缩、核染色质凝集边聚等特征,流式细胞术检测发现凋亡峰,DNA琼脂糖凝胶电泳可见明显的梯形条带。结论:土贝母皂苷使细胞周期阻滞和诱导细胞凋亡可能在其抗肿瘤作用中具有重要意义。
Background &Objective:Tubeimoside,which is composed of tubeimoside I (79%) and II (21%), was isolated from the tubers of Bolbostemma paniculatum (Maxim) Franquet (Cucurbitaceae), a traditional Chinese medicine, 'Tu Bei Mu'. This study was designed to investigate the anti tumor mechanism of tubeimoside. Methods:Growth inhibition was measured by MTT assay. Induction of cell cycle arrest and apoptosis was determined by flow cytometry, fluorescence and electron microscopy, and gel electrophoresis of fragmented DNA. Results: Tubeimoside display strong growth inhibitory effect in a dose and time dependant manner against HeLa cells with estimated IC50 values of 20.0, 18.8,and 8.8 μmol/L after 24,48,and 72 h of treatment with tubeimoside. The flow cytometry profiles revealed that treatment with tubeimoside (5 h;15,30,35 μmol/L) led to a dose dependant shift from 9 80%up to 21 90%,and 27 00%in percentage of cells with a G2/M like DNA content.On the other hand,treatment with tubeimoside(12 h,15,30,35 μmol/L)led to a time dependant shift from 8 20%up to 21 40%,31 15%,and 34 55%,respectively. Exposure of HeLa cells to 40 μmol/L of tubeimoside induced nuclear shrinkage,chromation condensation and margination against nuclear envelope,subdiploid peak,and DNA fragmentation, characteristic as seen in apoptotic cells. Conclusion: Induction of cell cycle arrest and apoptosis may play an important role in the anti tumor effect of tubeimoside.
出处
《癌症》
SCIE
CAS
CSCD
北大核心
2002年第4期346-350,共5页
Chinese Journal of Cancer
