摘要
观察左旋多巴神经毒性作用 ,探讨银杏叶提取物、左旋多巴联合治疗帕金森病大鼠可行性。采用帕金森病大鼠模型 ,通过细胞凋亡、免疫组织化学、行为学、 Nissl染色方法 ,选择帕金森病大鼠模型成功后 l0 d、 l2 d、 l4d为观察时点 ,比较左旋多巴 (L evodopa,L - dopa)治疗和银杏叶提取物 (Ginkgo biloba extract,EGb)、 L - dopa联合治疗(E- D组 )帕金森病大鼠的效果。结果 :与 L - dopa治疗组比较 ,E- D组大鼠黑质细胞凋亡数、旋转行为减少 ,而尼氏细胞计数、 b FGF蛋白表达则增加 (P<0 .0 5 )。表明 EGb可减轻 L - dopa的神经毒性作用 ,EGb、 L - dopa联合治疗帕金森病是可行的 ,且疗效优于单用 L -
The neuronal toxic effect of Levodopa was observed to investigate the feasibility of combined use of Levodopa and Ginkgo biloba extract (EGb) in the treatment of Parkinson disease (PD). The rat model of PD was made by injecting stereotaxically 6-OHDA to right side of the mesencephic ventral segmental area (VTA) and substantia nigra pars compacta (SNc). Rotational behaviour, TUNEL, immunocytochemical, Nissl's body staining methods were applied to observe the difference of therapeutic effects for PD between Levodopa (50 mg/kg every day×3 days, ×5 days, ×7 days, L-dopa group) and the combined use of Levodopa and EGb(100 mg/kg every day, E-D group). Results showed that the numbers of apoptosis and rotational behaviors were decreased, while bFGF protein expression and Nissl cell counts were increased in the E-D group as compared with those in the L-dopa group (P<0.05). It was suggested that EGb could alleviate the Levodopa-induced neuronal toxic effect. Combined use of EGb and L-dopa is feasible in the treatment of PD.
出处
《华中科技大学学报（医学版）》
CAS
CSCD
北大核心
2002年第2期174-177,共4页
Acta Medicinae Universitatis Scientiae et Technologiae Huazhong
基金
国家自然科学基金资助项目 (No.30 170 334 )
