摘要
探讨中药制剂和络舒肝胶囊的抗慢性肝损伤作用及作用机制。采用四氯化碳皮下注射 8周制作大鼠慢性肝损伤模型 ,用生理盐水配制的和络舒肝混悬液灌胃 ,2粒 / (只·次 ) ,每天 1次 ,连续 8周。观察肝组织病理变化 ,检测血清 AL T、AST、AKP、γ- GT值 ,放免法检测透明质酸 (HA)、层粘蛋白 (L N)、 型前胶原肽 (P P)和 型胶原水平 ,免疫组化检测肝组织转化生长因子 β1(TGFβ1)表达 ,RT- PCR检测肝组织 Smad3m RNA水平。结果 ,和络舒肝能减轻慢性肝损伤的组织病理改变 ,显著降低肝损伤大鼠血清 AL T、AST、AKP值及肝纤维化指标水平 ,P<0 .0 5或 P<0 .0 1。四氯化碳诱导的慢性肝损伤中 TGFβ1表达增加 ,使用和络舒肝胶囊可抑制肝组织中 TGFβ1蛋白表达。正常组、和络舒肝组以及模型组 Smad3m RNA表达亦明显不同 ,其转录水平呈现依次增高趋势。和络舒肝胶囊对大鼠慢性肝损伤具有保护作用 ,其作用机制可能与影响 TGFβ1蛋白表达水平有关 ,而且可能是通过抑制信号转导分子 Smad3m
The roles of the Chinese traditional medicine Heluoshugan capsule in rats' chronic hepatic injury and the mechanism were investigated. Rats' chronic hepatic injury model was induced by subcutaneous injection of CCl 4 for 8 weeks. Suspension of Heluoshugan preprared with normal saline was given orally to the rats, 2 capsules per rat every day for 8 weeks. Pahtological changes of liver were observed under light and electron microscope. The levels of serum ALT, AST, AKP and γ-GT were measured, serum levels of HA, LN, PⅢP and type Ⅳ collagen also detected by RIA, TGFβ1 expression in liver tissue by immunohistochemistry and Smad3 mRNA of liver tissue by PT-PCR. Results showed that Heluoshugan could improve the pathological changes of liver tissue, and significantly decreased the levels of ALT, AST, AKP, HA, LN, PⅢP and type Ⅳ collagen,P<0.05 or <0.01. TGFβ1 expression levels were increased in the rats with chronic hepatic injury. However, TGFβ1 expression was inhibited after administration of Heluoshugan. There were significant differences of Smad3 mRNA levels among all groups. The Smad3 mRNA level in the model group was higher than in the Heluoshugan group and normal control group. It was suggested Heluoshugan capsule might have protective effects on the chronic liver injury of rats by inhibiting the Smad3 mRNA transcriptional level of liver. The mechanism might be associated with the TGFβ1 expression level.
出处
《华中科技大学学报（医学版）》
CAS
CSCD
北大核心
2002年第2期171-173,177,222,共5页
Acta Medicinae Universitatis Scientiae et Technologiae Huazhong
基金
卫生部临床重点学科--内科肝脏病学建设基金资助项目 (No.0 7- 95 0 10 - 2 )
关键词
和络舒肝胶囊
大鼠
慢性肝损伤
保护作用
分子机制
研究
Chinese traditional medicine compound
Heluoshugan
chronic hepatic injury
pharmacology
