摘要
为了研究人脑恶性胶质瘤的细胞遗传学特征,我们采用G 带、R 带和C 带技术,对SHG—44细胞进行染色体分析。结果表明染色体分布从52～125,其中以超三倍体为主,占84.64%,亚三倍体占10.42%,超四倍体占4.94%。核型分析中以每对染色体的实际数与细胞倍体所期望的每对染色体数比较,分析各对染色体数目增加或减少,结果发现A、B 组染色体丢失,5~#减少明显,C 组7~#、11~#、12~#增加,其余各号减少,D、E、F、G 组各号均增加,17~#增加明显,以及性染色体的丢失。每次分析均可见3条较长的亚中部着丝点染色体,因外形异常,出现频率高,而且不随细胞传代消失,故我们认为是SHG—44的标记染色体。
G-,C-and R-banding technique were employed in the chromosome analysis of the hu-man malignant glioma cell line SHG-44 established in our laboratory.The chromosome numbersin the examined cells ranging from 52 to 125.The value of hypertripoid cells were 86.64%,while that of hypotriploid and hypertetraploid cell were 10.42% and 4.94%,respectively.Bycomparing the real number of each chromosome with ploicexpected number in the same cell,the losses and gains of each chromosome were determined.The losses of chromosomes were those ofgroup A and B(especially 5#)and 6#,8#,9#,15#,in group C and sex chromosome(s).The gains of chromosome were 7#,11#,12# in group C and those of group D,E,F,G(espe-cially 17#).Three big submetacentric chromosomes,observed with ratrer high frequencies,ex-isted in all the examined passages.These three chromosomes were considered to be the markerchromosomes of SHG-44.
出处
《苏州医学院学报》
1991年第3期179-181,254-255+259,共3页
Acta Academiae Medicinae Suzhou
关键词
人脑
恶性胶质瘤
染色体
human malignant glioma
chromosome.
