摘要
选用中国水仙鳞茎块离体培养,以叶大褐斑病原菌的培养滤液作为选择,结合γ-射线物理诱变,从1 420块外植体中得到9个抗病再生种球。抗病性测定表明,经病原菌培养滤液筛选处理得到的再生种球,抗病性比对照明显提高。
The bulbs from Narcissus tazetta var. chinensis were in vitro cultured. The fungal filtrate prepared from cultures of Stagonospora curtisii pathogenic bacteria was used as screening agent. 1 420 N. tazetta calli were treated with γ-ray mutation and 9 bulbils resistant to S.curtisii were produced. The result showed that the disease resistance of regenerated bulbils cultured on the selected medium is greater than that of the control.
出处
《亚热带植物科学》
2002年第1期25-27,共3页
Subtropical Plant Science
基金
福建省教委
科技项目基金资助(JB01065)
关键词
中国水仙
叶大褐斑病
抗病突变体
组织培养
离体筛选
Narcissus tazetta var. chinensis
Stagonospora curtisii
disease-resistant mutant
tissue culture
