摘要
In order to investigate the biological characteristics of the transgenic cellular model(HepG2.9706),the transgene copy numbers in HepG2.9706 were tested by complex probe fluorescent quantitative PCR and Southern hybridization.Its luciferase activity was assayed by luciferase assay system.The results showed that HepG2.9706,after consecutive passages for two years,keeps excellent luciferase activities and its transgene copy number is around 140 copies.This indicates that HepG2.9706 contains a high ratio of transgene integration and is very stable.
In order to investigate the biological characteristics of the transgenic cellular model(HepG2.9706),the transgene copy numbers in HepG2.9706 were tested by complex probe fluorescent quantitative PCR and Southern hybridization.Its luciferase activity was assayed by luciferase assay system.The results showed that HepG2.9706,after consecutive passages for two years,keeps excellent luciferase activities and its transgene copy number is around 140 copies.This indicates that HepG2.9706 contains a high ratio of transgene integration and is very stable.
出处
《病毒学报》
CAS
CSCD
北大核心
2002年第1期74-76,共3页
Chinese Journal of Virology
基金
国家"8 6 3"基金资助 (编号 :10 2 -0 8-0 4-0 1)
军队9 5重点课题 (编号 :96Z0 0 7)基金资助
