摘要
目的 :探讨横纹肌肉瘤 (RMS)中转化生长因子 β1(TGFβ1)及其Ⅰ、Ⅱ型受体 (TGFβRⅠ ,TGFβRⅡ )表达及其意义。 方法 :应用免疫组化技术 (S P法 )检测 4 9例经电镜或 (和 )免疫组化证实的横纹肌肉瘤及 2 0例正常横纹肌组织中的TGFβ1、TGFβRⅠ和TGFβRⅡ表达水平 ,并结合临床病理资料进行分析。 结果 :TGFβ1在RMS中的高表达率高于正常横纹肌组织 (P<0 0 1) ;TGFβRⅠ、TGFβRⅡ在 98% (48/ 4 9)的RMS及所有正常横纹肌组织中均有表达 ,两组无差异。TGFβ1表达与肌球蛋白重链呈负相关 (r=- 0 6 6 1,P <0 0 1)。TGFβ1在低分化组高表达率为 79% ,高分化组为 38%。 结论 :横纹肌肉瘤中存在TGFβ1自分泌和旁分泌现象 ,且肿瘤细胞表达相关受体。TGFβ1的表达与肌源性分化程度有关。
Purpose To investigate the expression of TGFβ1,and its receptorsⅠ, Ⅱ (TGFβRⅠ,TGFβRⅡ) in rhabdomyosarcoma (RMS), and their relationship with clinicopathological features. Methods Immunohistochemical technique(S P) was used to detect the expression of TGFβ1, TGFβRⅠ,RⅡ and myosin in 49 cases of rhabdomyosarcoma and 20 normal skeletal muscles. Results Increased expression of TGFβ1 was found in RMS compared with normal skeletal muscle ( P <0 01). Expression of TGFβ RⅠ and RⅡ were detected in most of RMSs and all of normal skeletal muscles without significant difference.There was a close negative correlation between the expression of TGFβ1 and myosin ( r =-0 661, P <0 01). Overexpression of TGFβ1 was oberserved in the low differentiated group more frequently than that in the high differentiated one. Conclusions There are TGFβ1 autocrine/paracrine pathway in RMS,with the expression of TGFβ1 associated receptors.Expression of TGFβ1 is related to the degree of myogenic differentiation of RMS. Overexpression of TGFβ1 may play a role in development and progression of rhabdomyosarcoma.
出处
《临床与实验病理学杂志》
CAS
CSCD
2002年第1期61-64,共4页
Chinese Journal of Clinical and Experimental Pathology
基金
纽约中华医学基金会 (CMB)基金资助项目 (No 0 0 72 2 )
关键词
横纹肌肉瘤
转化生长因子Β1
受体
免疫组织化学
rhabdomyosarcoma
transforming growth factor β
receptors,transforming growth factor β
immunohistochemistry
