摘要
目的应用超高效液相色谱-四级杆串联飞行时间质谱法(UPLC-Q-TOF-MS)对油炸马钱子提取物的主要化学成分进行定性研究。方法采用Agilent SB-C_(18)(2. 1 mm×100 mm,1. 8μm)色谱柱,以0. 1%甲酸水(A)-乙腈(B)溶液为流动相,梯度洗脱,流速为0. 2 m L·min^(-1),柱温为35℃;质谱分析采用信息关联模式(IDA)正、负离子分别采集。结果本实验共鉴定出油炸马钱子提取物中31个化学成分,其中有8个为在马钱子中首次报道,并在正、负离子模式下发现4个新化合物;主要成分包括生物碱、有机酸、糖苷等。结论该方法精确、可靠、高效,适用于油炸马钱子成分的快速鉴定,为马钱子炮制品的开发利用以及阐明其药效物质基础提供参考。
OBJECTIVE To identify the main chemical constituents in the extract of deep fried strychnas by UPLC-Q-TOF-MS.METHODS Agilent SB-C18(2.1 mm×100 mm,1.8μm)column was used.Gradient elution was conducted with mobile phase consisting of 0.1%formic acid solution(A)and acetonitrile(B)at a flow rate of 0.2 m L·min^-1.The column temperature was maitained at 35℃.MS analysis was based on information associated mode(IDA)that positive and negative ions were respectively collected.RESULTS A total of 31 compounds were identified in deep fried strychnas,of which eight had not been reported as Semen Strychni,and four new compounds were found in the positive and negative ion mode.The main chemical constituents included alkaloids,organic acids,glycosides,etc.CONCLUSION The method is accurate,reliable,and efficient,and is suitable for rapid identification of the ingredients in deep fried strychnas,which provides a reference for the development and utilization of processed products of Semen Strychni and clarification of its efficacy and material basis.
作者
秦伟瀚
阳勇
郭延垒
王云红
李卿
冉继春
QIN Wei-han;YANG Yong;GUO Yan-lei;WANG Yun-hong;LI Qing;RAN Ji-chun(Chongqing Academy of Chinese Materia Medica,Chongqing 400065,China)
出处
《中国药学杂志》
CAS
CSCD
北大核心
2019年第2期123-131,共9页
Chinese Pharmaceutical Journal
基金
重庆市科学技术委员会项目资助(cstcjxjl-jbky120009)
重庆市卫生计生委中医药科技项目资助(ZY201702142)
关键词
马钱子
油炸法
四级杆-飞行时间质谱
定性分析
裂解规律
Semen Strychni
frying method
quadrupole time-of-flight mass spectrometry (Q-TOF-MS)
qualitative analysis
cleavage law
