摘要
目的建立一种实验室检测产生碳青霉烯酶细菌的方法。方法取33株已知基因型产碳青霉烯酶细菌和108株临床分离细菌作为待测菌,利用亚胺培南水解法(Imipenem Hydrolysis Method,IHM)对细菌所产碳青霉烯酶进行检测。将涂有待测菌的纸片紧靠亚胺培南纸片依次贴在涂有0.5麦氏单位的大肠埃希菌(Escherichia coli)ATCC25922的MHA平板上,35℃培养,分别于8h、10h、24h观察亚胺培南纸片抑菌圈内细菌生长情况。同时利用改良碳青霉烯灭活试验(Modified Carbapenem Inactivation Method,mCIM)对待测菌进行检测,比较二者检测结果的一致性。结果 33株已知基因型产碳青霉烯酶细菌中,IHM检测全部阳性,mCIM 32株阳性,1株结果不确定,两种方法的检出率分别为100.00%和96.97%,比较差异无统计学意义(χ^2=1.015,P=0.314)。108株临床分离菌株,其中51株碳青霉烯敏感菌株两种方法检测均为阴性。57株碳青霉烯耐药菌株中,IHM 2株检测阴性,mCIM 3株检测阴性,共有5株细菌检测结果不同,两种检查方法进行一致性检测,Kappa值为0.823,一致性较好。结论与mCIM相比,IIM操作简单,耗时较短,可用于产碳青霉烯酶的肠杆科细菌的快速检测。
OBJECTIVE To establish a method for detection of carbapenemase-producing bacteria in laboratory.METHODS Totally 33 carbapenemase-producing strains with known genotypes and 108 clinical isolates of Enterobacteriaceae were chosen as the test strains,the carbapenemases produced by the strains were tested by usingimipenem hydrolysis method(IHM),the test sheets coated with the test strains were adjoined to imipenem sheet and successively stuck on MHA plate coated with 0.5 MCF of Escherichia coli ATCC25922 and were cultured with the temperature of 35℃,the bacterial growths in the inhibition zone of the imipenem sheets were observed after the culture of 8,10 and 24 hours.Meanwhile,the test strains were detected with the use of modified carbapenem inactivation method(mCIM).The consistency in detection of the strains was observed and compared between the detection method.RESULTS Of the 33 carbapenemase-producing strains with known genotypes,all were tested positive for IHM,32 were positive for mCIM,and 1 was unknown,the detection rates of the two methods were 100.00% and 96.97%,respectively,and there was no significant difference(χ^2=1.015,P=0.314).Of the108 clinical isolates,51 were tested negative for the both detection methods.Of 57 carbapenem-resistant strains,2 were tested negative for IHM,3 were tested negative for mCIM,totally 5 strains showed the different test results,and the consistency test of the two detection methods indicated that the Kappa coefficient was 0.823 and showed good consistency.CONCLUSIONAs compared with mCIM,IHM is simple and time-saving and can be used for the rapid detection of the carbapenemase-producing strains.
作者
蔡辉
凌耿飞
周战修
陈露露
王艳侠
姜梅杰
CAI Hui;LING Geng-fei;ZHOU Zhan-xiu;CHEN Lu-lu;WANG Yan-xia;JIANG Mei-jie(Affiliated Jinxiang Hospital of Jining Medical College,Jining,Shandong 272200,China)
出处
《中华医院感染学杂志》
CAS
CSCD
北大核心
2019年第1期15-19,共5页
Chinese Journal of Nosocomiology
基金
山东省自然科学基金资助项目(22011HM036)
济宁市科技计划基金资助项目(2016-56-78)
