摘要
目的评价丙泊酚麻醉与老龄大鼠术后谵妄(POD)及炎症反应的关系。方法老年雄性SD大鼠24只,18月龄,体重550~650g,采用随机数字表法分为3组(n=8):对照组(C组)、丙泊酚麻醉2h组(P2组)和丙泊酚麻醉4h组(P4组)。P2组和P4组采用丙泊酚进行诱导麻醉后,以24mg·kg^-1·h^-1的速率分别维持麻醉2和4h,并于麻醉前1d和麻醉后1d行Morris水迷宫实验。水迷宫测试结束后,采集血液和脑脊液标本,采用酶联免疫吸附法测定血浆和脑脊液IL-1β、IL-4、IL-6和TNF-α的浓度;取海马和皮层组织,计数Iba-1阳性细胞数。结果与C组和麻醉前比较,P2组和P4组麻醉后1d逃避潜伏期和原平台象限停留时间百分比差异无统计学意义(P>0.05),P2组脑脊液IL-6浓度和血浆TNF-α浓度降低,P4组脑脊液IL-1β浓度和血浆IL-4、IL-6和TNF-α浓度升高,血浆IL-1β浓度和脑脊液IL-4浓度降低,P2组和P4组海马Iba-1阳性细胞数减少(P<0.05或0.01)。结论丙泊酚麻醉4h内未能诱发老龄大鼠POD及中枢炎症反应;麻醉时间长于2h可诱发外周炎症反应。
Objective To evaluate the relationship between propofol anesthesia and postoperative delirium and inflammatory responses of aged rats. Methods Twenty-four adult male Sprague-Dawley rats, aged 18 months, weighing 550-650 g, were divided into 3 groups (n=8 each) using a random number table method: control group (group C), propofol anesthesia for 2 h group (group P2) and propofol anesthesia for 4 h group (group P4). In P2 and P4 groups, anesthesia was maintained with propofol at a rate of 24 mg·kg^-1·h^-1 for 2 and 4 h, respectively, after anesthesia was induced with propofol.Morris water maze test was performed at 1 day before and after anesthesia.Blood and cerebrospinal fluid samples were collected after the end of Morris water maze test for determination of interleukin-1beta (IL-1β), IL-4, IL-6 and tumor necrosis factor-alpha (TNF-α) concentrations in plasma and cerebrospinal fluid by enzyme-linked immunosorbent assay.The hippocampus and cortex were removed for determination of Iba-1 positive cells. Results Compared with group C and the baseline value before anesthesia, no significant change was found in the escape latency or percentage of time of staying at the original platform quadrant in P2 and P4 groups (P>0.05), the concentrations of IL-6 in cerebrospinal fluid and TNF-α in plasma were significantly decreased in group P2, the concentrations of IL-1β in cerebrospinal fluid and IL-4, IL-6 and TNF-α in plasma were significantly increased, and the concentrations of IL-1β in plasma and IL-4 in cerebrospinal fluid were decreased in group P4, and the number of hippocampal Iba-1 positive cells were significantly decreased in P2 and P4 groups (P<0.05 or 0.01). Conclusion Propofol anesthesia dose not induce postoperative delirium and central inflammatory responses within 4 h, and propofol can induce peripheral inflammatory responses when anesthesia time is longer than 2 h.
作者
朱姣
桂潇
李文迁
陆智杰
Zhu Jiao;Gui Xiao;Li Wenqian;Lu Zhijie(Department of Anesthesiology, Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, Shanghai 200438, China)
出处
《中华麻醉学杂志》
CAS
CSCD
北大核心
2018年第10期1198-1200,共3页
Chinese Journal of Anesthesiology
基金
上海市科委项目(17411960400).
