摘要
目的通过体内及体外实验,初步探究欧前胡素(imperatorin,IMP)在肥大细胞脱颗粒中的作用机制。方法建立大鼠被动皮肤过敏反应(passive cutaneous anaphylaxis,PCA)模型,采用比色测定法检测IMP在体内对肥大细胞的影响;通过体外实验观察欧前胡素在肥大细胞的组胺释放、钙内流、肿瘤坏死因子-α(TNF-α)和白细胞介素-6(IL-6)释放中的作用,以及对Pi3k/Akt和NF-κB信号通路的影响。结果体内实验结果显示,欧前胡素(15 mg·kg^(-1),30 mg·kg^(-1),60 mg·kg^(-1))明显抑制大鼠过敏反应。体外实验结果表示,欧前胡素(10μmol·L^(-1)、20μmol·L^(-1)、40μmol·L^(-1))显著减少肥大细胞中TNF-α、IL-6的含量以及组胺释放和钙内流,并且抑制NF-κB p65蛋白的表达。结论 IMP的抗过敏作用可能部分是通过抑制Pi3k/Akt和NF-κB信号通路,进而减少肥大细胞释放组胺、钙内流,降低TNF-α、IL-6等炎症因子的水平而实现的。
To investigate the roles of imperatorin(IMP) in mast cell degranulation and its mechanism in vivo and in vitro, colorimetric assay was employed to detect the influence of IMP on the rats of PCA model, we also observed the effects of IMP on histamine release, calcium influx, TNF-α and IL-6 release in mast cells, as well as the effects on Pi3k/Akt and NF-κB signaling pathways. In vivo experimental data showed that IMP(15 mg·kg^-1,30 mg·kg^-1, 60 mg·kg^-1) significantly inhibited allergic reactions in rats;in vitro data indicated that IMP(10 μmol·L-1, 20 μmol·L-1, 40 μmol·L-1) significantly reduced TNF-α and IL-6 levels, histamine release and calcium influx of mast cells, and inhibited the expression of NF-κB p65 protein. Taken together, the anti-allergic effect of IMP may be partially achieved by inhibiting the Pi3k/Akt and NF-κB signaling pathways, thereby reducing the histamine and calcium influx from mast cells and reducing the levels of inflammatory factors such as TNF-α, IL-6 and so on.
作者
咸哲民
王重阳
姜京植
朱莲花
金珊
金哲虎
李俊峰
李良昌
朴红梅
延光海
XIAN Zhemin;WANG Chongyang;JIANG Jingzhi;ZHU Lianhua;JIN Shan;JIN Zhehu;LI Junfeng;LI Liangcang;PIAO Hongmei;YAN Guanghai(Department of Respiratory Medicine,Yanbian University Hospital,Yanji 133000,China;Department of Anatomy, School of Basic Medical Sciences,Yanbian University,Yanji 133000,China;Department of Dermatology,Yanbian University Hospital,Yanji 133000,China)
出处
《免疫学杂志》
CAS
CSCD
北大核心
2019年第2期126-131,共6页
Immunological Journal
基金
国家自然科学基金(81660003
81560679)
