摘要
目的 评价榭皮素预先给药对全脑缺血再灌注大鼠血脑屏障通透性的影响.方法清洁级健康雄性SD大鼠63只,4~5月龄,体重300~350 g,采用随机数字表法分为3组( n=21):假手术组(S组)、缺血再灌注组(I∕R组)和榭皮素预先给药组(Q组).采用夹闭双侧颈总动脉联合低血压法制备大鼠全脑缺血再灌注模型. Q组于制备模型前3 d腹腔注射榭皮素25 μmol∕kg,2次∕d,连续3 d. S组和I∕R组分别于相应时点腹腔注射等体积生理盐水.于再灌注24 h时处死大鼠取脑,干湿重法测定脑含水量,荧光显微镜下观察脑组织对伊文思蓝( EB)的通透性,透射电镜下观察血脑屏障超微结构,Western blot法检测脑皮质occludin蛋白表达水平.结果 与S组比较,I∕R组和Q组大鼠脑含水量和EB含量升高,occludin蛋白表达下调(P<0. 05),血脑屏障超微结构损伤加重;与I∕R组比较,Q组大鼠脑含水量和EB含量降低,occludin蛋白表达上调(P<0. 05),血脑屏障超微结构损伤减轻.结论 榭皮素预先给药可降低全脑缺血再灌注后血脑屏障通透性,减轻脑水肿,其机制可能与上调occludin蛋白表达有关.
Objective To evaluate the effect of quercetin pretreatment on the permeability of blood-brain barrier in a rat model of global cerebral ischemia-reperfusion ( I∕R). Methods Sixty-three clean-grade healthy male Sprague-Dawley rats, weighing 300-350 g, aged 4-5 months, were divided into 3 groups (n=21 each) using a random number table method: sham operation group ( group S), group I∕R and quercetin pretreatment group ( group Q). Global cerebral I∕R was induced by occlusion of bilateral common carotid arteries combined with hypotension ( mean arterial pressure was maintained at 35-45 mmHg) in chloral hydrate-anesthetized rats. Quercetin 25 μmol∕kg was injected intraperitoneally twice a day for 3 consecutive days starting from 3 days before establishment of the model in group Q, while the e-qual volume of normal saline was given instead at the corresponding time points in group S and group I∕R, respectively. The animals were sacrificed at 24 h of reperfusion and brains were removed to determine the brain water content, Evans blue ( EB) content and expression of occludin protein in cerebral cortex ( by Western blot) and to observe the ultrastructure of blood-brain barrier. Results Compared with group S, the brain water content and EB content were significantly increased, the expression of occludin protein was down-regulated (P<0. 05), and the injury to ultrastructure of blood-brain barrier was accentuated in I∕R and Q groups. Compared with group I∕R, the brain water content and EB content were significantly de-creased, the expression of occludin protein was up-regulated (P<0. 05), and the injury to ultrastructure of blood-brain barrier was significantly attenuated in group Q. Conclusion Quercetin pretreatment can de-crease the permeability of blood-brain barrier and attenuate brain edema, and the mechanism may be related to up-regulated expression of occludin protein in a rat model of global cerebral I∕R.
作者
金朝
吴会生
郭培培
柯剑娟
李心怡
张宗泽
王焱林
冯晓波
Jin Zhao;Wu Huisheng;Guo Peipei;Ke Jianjuan;Li Xinyi;Zhang Zongze;Wang Yanlin;Feng Xiaobo(Department of Anesthesiology,Zhongnan Hospital,Wuhan University,Wuhan 430071,China)
出处
《中华麻醉学杂志》
CAS
CSCD
北大核心
2018年第7期866-869,共4页
Chinese Journal of Anesthesiology
基金
国家自然科学基金(81471858)
湖北省卫生计生科研基金(WJ2017M036)。
关键词
黄酮类
脑
再灌注损伤
血脑屏障
Quercetin
Cerebral
Ischemia-reperfusion
Biood-brain barrier
