摘要
为探究重组禽流感病毒(H5+H7)二价灭活疫苗对鹅的免疫特性,将100只27日龄四季鹅,随机分成3组:一免组40只,接种剂量0.5 mL/只;二免组40只,二次接种剂量分别为0.5 mL/只、1.5 mL/只;对照组20只,不免疫。结果显示:一免后21 d H5(Re-8株)免疫抗体平均滴度为5.85 log2,抗体合格率为85%,28 d为7 log2,抗体合格率为100%,但60 d后平均滴度降为5.95 log2;二免后7 d为7.15 log2,39 d为7.95 log2,抗体合格率均保持100%。一免后21 d H7N9免疫抗体平均滴度为5.2 log2,抗体合格率为85%,28 d为6.6 log2,抗体合格率达到100%,60 d后降为3.15 log2;二免后7 d为6.8 log2,39 d为7.05 log2,抗体合格率均保持在100%。结果表明:该疫苗能够有效刺激鹅产生免疫保护抗体,适用于鹅的禽流感免疫;为增强免疫保护效果,建议进行二次免疫。
In order to explore the immune characteristics of the bivalent inactivated vaccine of recombinant avian influenza virus (H5+H7) in geese, 100 geese at the age of 27 days were randomly divided into 3 groups, group one, 40 geese (immunized once, the immunization dose was 0.5 mL per goose) ; group two, 40 geese (immunized twice, the immunization dose at first and second immunization were 0.5 mL and 1.5 mL per goose respectively); group three (control group) , 20 geese. Results showed that, for the antibodies against H5 subtype virus (Re-8 strain) , the average antibody titer was 5.85 log2 at 21st day after the first immunization, the qualified rate was 85%; at 28th day, the antibody titer was 7 log2, and the qualified rate was 100%. However, the average titer decreased to~'5.95 Iog2 after 60 days. At 7th and 39th day after second immunization, the average antibody titer were 7.15 log2 and 7.95 log2, respectively, and the qualified rates were all 100%. For the antibodies against H7N9 virus, the average antibody titer was 5.2 log2 at 21st day after the first immunization, the qualified rate was 85%; at 28th day, the antibody titer was 6.6 log2, and the qualifiedrate was 100%. And the antibody titer decreased to 3.15 log2 after 60 days. Similarly, at 7th and 39th day after second immunization, the average antibody titer were 6.8 log2 and 7.05 log2, respectively, and the qualified rates were all 100%. As a conclusion, the inactive vaccine could effectively stimulate the geese to produce protective antibodies, which was suitable for geese immunization. For the purpose of enhancing the immune protection effects, it was recommended to carry out second immtmization.
作者
徐正军
邱冬
董永毅
吴瑛
李瑛
王相子
徐小艳
开妍
蒋彩霞
Xu Zhengjun;Qiu Dong;Dong Yongyi;Wu Ying;Li Ying;Wang Xiangzi;Xu Xiaoyan;Kai Yan;Jiang Caixia(Jiangsu Animal Diseases Prevention and Control Center, Nanjing, Jiangsu 210036, China;Nanjing Agricultural University, Nanjing, Jiangsu 210095, China;Zhenjiang Animal Diseases Prevention and Control Center, Zhenjiang, Jiangsu 212009, China;MaliAgro-ecologicalGarden, Jurong, Jiangsu 212403, China)
出处
《中国动物检疫》
CAS
2018年第11期62-65,共4页
China Animal Health Inspection
基金
江苏省农业三新工程项目(SXGC[2017]232)
关键词
免疫试验
重组禽流感病毒(H5+H7)二价灭活疫苗
鹅
immune experiment
bivalent inactivated vaccine of recombinant H5 and H7 subtype avian influenza vires
goose
