摘要
孔隙部分是离子通道选择性控制的关键部分 与Ｋ＋通道相似，ＨＣＮ离子通道孔隙区域包括ＧＹＧ特征序列 ．但除了Ｋ＋外，Ｎａ＋也能选择性通过ＨＣＮ离子通道 对ｍＨＣＮ１孔隙多肽的构象研究将有助于对ＨＣＮ离子通道选择性的理解． 本文利用新开发的ＷＥＴ溶剂抑制方法并综合运用ｗｅｔ－ＴＯＣＹＳ和ｗｅｔ－ＮＯＥＳＹ ＮＭＲ方法对ｍＨＣＮ１孔隙多肽（９５％水＋５％重水）进行了１Ｈ谱线归属．
Like K+ channel, the pore region of HCN channel includes GYG characteristic se- quence. But besides K+, Na + can selectively overpass HCN channel. The conformation study of mHCN1 pore region peptide helps to understand the selectivity of HCN channel. In this article, new developed WET sequence was used to suppress solvent peak. With the com- prehensive use of wet-TOCSY and wet-NOESY NMR experiments, the 1H resonance of mHCN1 pore region peptide was assigned. (95%H2O+ 5%D2O).
出处
《波谱学杂志》
CAS
CSCD
北大核心
2002年第1期33-40,共8页
Chinese Journal of Magnetic Resonance
