摘要
目的 探讨非小细胞肺癌 (NSCLC)Fas蛋白表达与DNA的关系。方法 对 33份手术切除的NSCLC标本和 17份正常肺组织使用流式细胞术检测Fas蛋白表达和DNA含量 ,并与细胞增殖活性、增殖指数 (PI)、S期分数 (SPF)以及临床病理特征之间进行比较分析。结果 NSCLC的异倍体率为82 % ,DNA指数 (DI)及PI、SPF分别为 1 36± 0 2 3,0 2 9± 0 0 9,0 144± 0 0 78,与正常肺组织相比差异有极显著性 (P =0 0 0 0 )。在NSCLC中Fas的表达低于正常肺组织 (P =0 0 0 0 ) ,并显示与PI、SPF呈负相关 (P分别为 0 0 2 4、0 0 0 4)。Fas表达及细胞增殖活性与NSCLC的临床病理特征如年龄、肿瘤大小、淋巴结转移、病理类型之间未见明显相关。结论 NSCLC的Fas表达降低和细胞增殖活性增高具有普遍意义 ;Fas表达的下降影响了NSCLC细胞的增殖活性 ,这种增殖与凋亡的调控异常在NSCLC发病中可能起重要作用。
Objective To study the relationship between Fas protein expression and DNA content in non-small cell lung cancer (NSCLC). Methods Fas protein expression and DNA content in resected samples from 33 NSCLC patients and 17 normal controls were detected by flow cytometry. Fas expression and DNA content were compared with cell proliferation activity which was defined by proliferation index and s-phase fraction (SPF), and with clinicopathological parameters.Results The percentage of DNA aneuploidy in NSCLC was 82%. The DNA index (DI),PI and SPF in NSCLC were 1.36±0.23, 0.29±0.09 and 0. 144±0.078 respectively,which were significantly higher than those in normal lung tissue.The Fas protein expression was lower in NSCLC than that in normal tissue( P =0.000) and showed negative correlation with PI ( P =0.024) and SPF( P =0.004). Fas expression and the cell proliferation activity were not correlated to the clinicopathological parameters including age, tumor size,lymph node metastasis and the histological types of lung cancer ( P >0.05). Conclusions Decrease of Fas protein expression and increase of proliferation activity are common in NSCLC. The reduction of Fas protein expression may affect the proliferation activity of NSCLC.This abnormal regulation of apoptosis and proliferation may play an important role in the pathogenesis of NSCLC.
出处
《中华结核和呼吸杂志》
CAS
CSCD
北大核心
2002年第4期210-213,共4页
Chinese Journal of Tuberculosis and Respiratory Diseases
