摘要
目的 :探讨中药透皮治疗哮喘的作用机制。方法 :采用Hutson法豚鼠哮喘模型 ,治疗组隔日诱喘后用中药敷贴 30min ,对照组诱喘后隔日采用 9g/L氯化钠溶液浸润纱布或 0 .5 g/L醋酸地塞米松霜敷贴 ,比较记录诱喘潜伏期 ;2周后处死 ,取肺组织 ,用放射配基竞争结合法比较M受体表达状况。结果 :中药透皮治疗能显著延长哮喘豚鼠诱喘潜伏期 ,减轻发作程度 ,下调哮喘豚鼠肺组织M受体含量。结论 :2次实验结果提示 ,降低M受体含量是中药循经敷贴治疗哮喘的重要机制之一。
Objective:To investigate the mechanism of herbal penetration therapy for allergic asthma. Method:The asthma guinea pig models induced by Huston′s methods were externally applied with herb for 30 min quaque omni die. A control group of guinea pigs were applied with wet gauzes in 9 g/L of sodium chloride or 0.5 g/L of dexamethasone acetate paste in the same method. The incubation period of causing asthma was recorded and compared with each other. They were killed after two weeks. The muscarinic receptor expression in lung tissues was observed by radioligand binding assay. Result: The incubation period of causing asthma was prolonged significantly,the asthmatic attacks alleviated,and the muscarinic receptor expression down regulated. Conclusion:Down regulation of the muscarinic receptor expression in lung tissues is an important mechanism of herbal penetration therapy for allergic asthma.
出处
《安徽中医学院学报》
CAS
2002年第2期47-49,共3页
Journal of Anhui Traditional Chinese Medical College
基金
上海市科学技术发展基金资助项目 (984 0 190 2 9)
关键词
哮喘
M乙酰胆碱能受体
中药透皮疗法
秦氏哮喘膏
Asthma
Muscarinic cholinergic receptor
Herbal penetration therapy
Qinshi Xiaochuan plaster
