摘要
对东湖异养细菌群落结构采用系统分类和微机聚类分析方法作了比较研究。169株由东湖Ⅰ、Ⅱ、Ⅲ站水体中分离的异养细菌分别隶属于30个细菌属。其中以芽孢杆菌属居于明显优势,其次是微球菌属。在不同水域或水层,细菌的分布表现有一定的差别,但各站间多样性指数值没有明显的差异。基于113项生物学特性检测结果,采用lBM PC/XT微机聚类分析后计算各站细菌的多样性指数发现:Ⅰ站明显高于Ⅱ、Ⅲ两站,43项检测系统所得结果与此表现了一致的趋势。这与东湖各站水环境的理化分析参数是比较吻合的。作者对以上结果作了讨论。
The purpose of this study is to describe community structure of heterotrophic bactera in the Donghu Lake (Wuhan, Hubei, P. R. China) and compare empirical taxonomy and cluster analysis methods. A total of 169 bacterial strains growing on nutrient agar plates was isolated from water samples at different stations and water depths. Each isolate was identified by empirical taxonomy. The results indicated that they belong to Achromobacter, Acidaminococcus, Acinetobacter, Aerocoecus, Aeromonas, Alcaligenes, Bacillus, Beneckea, Brevibacterium, Citrobacter, Clostridium, Corynebacterium, Enterobacter, Erwinia, Escherichia, Flavobacterium, Gaffkya, Micrococcus, Microbacterium, Neisseria, Pediococcus, Planococcus, Pseudomonas, Sarcina, Serrtia, Siderocopsa, Siderococcus, Staphylococcus, Streptococcus, and Zymobacterium 30 genera respectively, except for 2 cocci unidentified. The Bacillus was dominanting by number, especially, in the water sample from Station 1, it overran 50% in all strains. It showed that some bacterial groups distribution was different, but the strains of Bacillus, Micrococcus, Acinetobacter, Brevibacterium, Enterobacter, Flavobacterium, and Staphylococcus could be found in all stations. The diversity index of bacterial genus at three stations was approximate to each other. (See tab.4). A cluster analysis by computer IBM PC/XT was used for studying heterotrophic bacterial community structure of the Donghu Lake. Each isolate was examined by conventional techniques for 113 morphological, cultural, biochem-physiological and antibiotic resistance characteristics and by a set of 43 biochem-physiological test system. All data were clustered by computer according to the similarity value. The results indicated the diversity index at Station 1 was higher than those at the Station Ⅱ and Ⅲ, although the diversity index value from the 43 system was lower than 113 system (see tab.4). Comparison of water quality including the concentration of NO_2, NO_3, NH_3, inorganic N, SiO_2, total P and N/P showed that the water quality at Station Ⅱ and Ⅲ was similar but was quite different from the Station Ⅰ. (Tab. 3). These data were consistent with the cluster analysis results. In other words, the diversity index from cluster analysis coincided with environmental conditions. Based on above mentioned, it seems that cluster analysis is easier to do than conventional classification. Becouse identification of bacteria is extremely laborious and uncertain due to the geart variability of bacteria, so that that is very difficult in most cases. The chief advantage of cluster analysis is an objective description of individuals, we can get much more information on each strain and consider them as a set of abilities, which can be concerned with thier environment, thus the method may be especially useful in ecological studies.
出处
《水生生物学报》
CAS
CSCD
北大核心
1991年第3期242-254,共13页
Acta Hydrobiologica Sinica
基金
国家自然科学基金 编号3860591
关键词
异养细菌
群落结构
聚类分析
Heterotrophic bacterial community structure
Cluster analysis
